Light-Controlled Membrane Mechanics and Shape Transitions of Photoswitchable Lipid Vesicles
Light-Controlled Membrane Mechanics and Shape Transitions of Photoswitchable Lipid Vesicles
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DOI：
10.1021/acs.langmuir.7b01020
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发表时间：
2017-04-25
期刊：
影响因子：
3.9
通讯作者：
Lohmueller, Theobald
中科院分区：
文献类型：
--
作者：
Pernpeintner, Carla;Frank, James A.;Lohmueller, Theobald
巨单层囊泡（GUV）代表了一个通用的模型系统，以模拟与活细胞质膜相关的基本性质和功能。脂质囊泡的变形性和形状转变与双层膜本身的机械性质密切相关，并且通常难以在生理条件下控制。在这里，我们开发了一个协议，以形成细胞大小的囊泡从偶氮苯含磷脂酰胆碱（偶氮PC），经历光异构化与UV-A和可见光照射。光脂囊泡内的光开关能够快速和精确地控制膜的机械性能。通过改变光刺激的强度和动力学，实现了受控的囊泡形状变化，如出芽过渡、内陷、珠光化或膜管的形成。有了这个系统，我们可以在没有任何与细胞骨架相关的分子机器的情况下，模拟细胞中常见的形态变化。此外，我们设计了一种机制，利用光开关脂质膜来存储机械能，然后根据命令将其释放为本地可用的功。
Giant unilamellar vesicles (GUVs) represent a versatile model system to emulate the fundamental properties and functions associated with the plasma membrane of living cells. Deformability and shape transitions of lipid vesicles are closely linked to the mechanical properties of the bilayer membrane itself and are typically difficult to control under physiological :conditions. Here, we developed a protocol to form cell-sized vesicles from an azobenzene-containing phosphatidylcholine (azo-PC), which undergoes photoisomerization on irtadiation with UV-A and visible light. Photo switching within the photolipid vesicles enabled rapid and precise control of the mechanical properties of the membrane. By varying the intensity and dynamics of the optical stimulus, controlled vesicle shape changes such as budding transitions, invagination, pearling, or the formation of membrane tubes were achieved. With this system, we could mimic the morphology changes normally seen in cells, in the absence of any molecular machines associated with the Cytoskeleton. Furthermore, we devised a mechanism to utilize photoswitchable lipid membranes for storing mechanical energy and then releasing it on command as locally usable, work.