A nanocompartment system contributes to defense against oxidative stress in Mycobacterium tuberculosis.
A nanocompartment system contributes to defense against oxidative stress in Mycobacterium tuberculosis.
复制标题
纳米隔室系统有助于结核分枝杆菌防御氧化应激。
DOI：
10.7554/elife.74358
复制
发表时间：
2021-11-09
期刊：
影响因子：
7.7
通讯作者：
Stanley SA
中科院分区：
文献类型：
--
作者：
Lien KA;Dinshaw K;Nichols RJ;Cassidy-Amstutz C;Knight M;Singh R;Eltis LD;Savage DF;Stanley SA
囊蛋白纳米区室是一类新兴的原核蛋白质基细胞器，由包封蛋白质货物的囊蛋白质壳组成。编码纳米区室的基因广泛存在于细菌和古细菌中，最近的工作已经表征了几种货物酶的生化功能。然而，这些细胞器对宿主生理的重要性知之甚少。在这里，我们报告说，人类病原体结核分枝杆菌（Mtb）产生的纳米室，其中包含染料脱色过氧化物酶DyP。我们表明，这种纳米区室对于结核分枝杆菌在低pH环境中抵抗氧化应激的能力是重要的，包括在宿主细胞感染期间和用临床相关抗生素治疗后。我们的研究结果首次揭示了细菌发病机制中的纳米区室，并揭示了结核分枝杆菌用于对抗氧化应激的新机制。
Encapsulin nanocompartments are an emerging class of prokaryotic protein-based organelle consisting of an encapsulin protein shell that encloses a protein cargo. Genes encoding nanocompartments are widespread in bacteria and archaea, and recent works have characterized the biochemical function of several cargo enzymes. However, the importance of these organelles to host physiology is poorly understood. Here, we report that the human pathogen Mycobacterium tuberculosis (Mtb) produces a nanocompartment that contains the dye-decolorizing peroxidase DyP. We show that this nanocompartment is important for the ability of Mtb to resist oxidative stress in low pH environments, including during infection of host cells and upon treatment with a clinically relevant antibiotic. Our findings are the first to implicate a nanocompartment in bacterial pathogenesis and reveal a new mechanism that Mtb uses to combat oxidative stress.