CUP-SHAPED COTYLEDON1 transcription factor activates the expression of LSH4 and LSH3, two members of the ALOG gene family, in shoot organ boundary cells
CUP-SHAPED COTYLEDON1 transcription factor activates the expression of LSH4 and LSH3, two members of the ALOG gene family, in shoot organ boundary cells
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DOI：
10.1111/j.1365-313x.2011.04571.x
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发表时间：
2011-06-01
期刊：
影响因子：
7.2
通讯作者：
Aida, Mitsuhiro
中科院分区：
文献类型：
--
作者：
Takeda, Seiji;Hanano, Keiko;Aida, Mitsuhiro
器官边界的建立是多细胞生物正确形态发生的基本过程。在植物中，茎分生组织从其外围重复形成器官原基，它们之间产生边界细胞以分隔它们的细胞命运。CUP-SHAPED COTYLEDON 1（CUC 1）和CUC 2基因编码植物特异性NAC转录因子，在拟南芥茎器官边界的建立中起着核心作用。在这里，我们表明，CUC 1蛋白激活表达的光依赖性短下胚轴4（LSH 4）和它的同系物LSH 3在拍摄器官边界细胞。这两个基因编码拟南芥LSH 1和水稻G1（ALOG）家族的核蛋白，其成员在陆地植物中广泛保守。LSH 4和LSH 3的表达在各种芽器官（如子叶、叶和花器官）的边界细胞中检测到，并且需要CUC 1和CUC 2的活性。使用糖皮质激素受体系统的实验表明，LSH 4和LSH 3的转录直接由CUC 1上调。LSH 4在茎尖中的组成型表达导致营养期叶生长的抑制，以及生殖期花内额外的茎或茎器官的形成。总之，我们的研究结果表明，CUC 1直接激活核因子基因LSH 4和LSH 3的转录，这可能会抑制边界区域的器官分化。
The establishment of organ boundaries is a fundamental process for proper morphogenesis in multicellular organisms. In plants, the shoot meristem repetitively forms organ primordia from its periphery, and boundary cells are generated between them to separate their cellular fates. The genes CUP-SHAPED COTYLEDON1 (CUC1) and CUC2, which encode plant-specific NAC transcription factors, play central roles in establishment of the shoot organ boundaries in Arabidopsis thaliana. Here we show that CUC1 protein activates expression of LIGHT-DEPENDENT SHORT HYPOCOTYLS 4 (LSH4) and its homolog LSH3 in shoot organ boundary cells. Both genes encode nuclear proteins of the Arabidopsis LSH1 and Oryza G1 (ALOG) family, the members of which are widely conserved in land plants. Expression of LSH4 and LSH3 is detected in the boundary cells of various shoot organs, such as cotyledons, leaves and floral organs, and requires the activity of CUC1 and CUC2. Experiments using the glucocorticoid receptor system indicate that transcription of LSH4 and LSH3 is directly up-regulated by CUC1. Constitutive expression of LSH4 in the shoot apex causes inhibition of leaf growth in the vegetative phase, and formation of extra shoots or shoot organs within a flower in the reproductive phase. Together, our results indicate that CUC1 directly activates transcription of the nuclear factor genes LSH4 and LSH3, which may suppress organ differentiation in the boundary region.