Two different fucosylated chondroitin sulfates: Structural elucidation, stimulating hematopoiesis and immune-enhancing effects
Two different fucosylated chondroitin sulfates: Structural elucidation, stimulating hematopoiesis and immune-enhancing effects
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两种不同的岩藻糖基化硫酸软骨素：结构阐明、刺激造血和免疫增强作用
DOI：
10.1016/j.carbpol.2019.115698
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发表时间：
2020-02-15
影响因子：
11.2
通讯作者：
Yu, Guangli
中科院分区：
文献类型：
--
作者：
Niu, Qingfeng;Li, Guoyun;Yu, Guangli
分别从海参和管状海参中分离得到两种岩藻糖基化硫酸软骨素FCShp 和FCSht。应用核磁共振波谱和 HILIC-FTMS 方法对其进行详细的结构表征。化学分析表明，两种FCS均含有硫酸软骨素主链硫酸软骨素样核心和与葡萄糖醛酸残基O-3连接的α-L-Fuc2,4S、α-L-Fuc4S或α-L-Fuc3,4S的岩藻糖基分支。 FCShp和FCSht的主要分支是单岩藻糖，还检测到少量具有α-1,3-连接类型的二岩藻糖、三岩藻糖和四岩藻糖。最后，我们研究了 FCShp 和 FCSht 在环磷酰胺 (CTX) 诱导的免疫抑制小鼠模型中的免疫调节功能。结果表明，FCShp和FCSht对CTX诱导的骨髓抑制小鼠的造血功能恢复具有有益作用。值得注意的是，α-L-Fuc2,4S 对活性的影响比 α-L-Fuc3,4S 更重要。这些结果为开发减少化疗副作用的药物提供了基础。
Two fucosylated chondroitin sulfates FCShp and FCSht were isolated from the sea cucumber Holothuria polii and Holothuria tubulosa, respectively. The NMR spectroscopy and HILIC-FTMS methods were applied for their detailed structural characterization. Chemical analysis indicated that the two FCSs all contained a chondroitin sulfate backbone chondroitin sulfate-like core and fucosyl branches of alpha-L-Fuc2,4S, alpha-L-Fuc4S or alpha-L-Fuc3,4S linked to O-3 of glucuronic acid residues. The main branches of FCShp, and FCSht were monofucose, and the small amounts of di-, tri- and tetrafucose with alpha-1,3-linkage type were also detected. Finally, we investigated the immunomodulatory function of FCShp, and FCSht in cyclophosphamide (CTX)-induced immunosuppressed mouse models. The results showed that FCShp, and FCSht had beneficial effects on hematopoietic function recovery in CTX-induced bone marrow suppression mice. Notably, the alpha-L-Fuc2,4S was more important to the activity than alpha-L-Fuc3,4S. These results provided basis for developing the drugs to reduce side effects of chemotherapy.