Interplay of permanent charge and hydrophobicity in the electrospray ionization of glycans.
Interplay of permanent charge and hydrophobicity in the electrospray ionization of glycans.
复制标题
DOI：
10.1021/ac101227a
复制
发表时间：
2010-08-01
影响因子：
7.4
通讯作者：
Muddiman DC
中科院分区：
文献类型：
--
作者：
Walker SH;Papas BN;Comins DL;Muddiman DC
由于N-连接的葡聚糖具有亲水性和缺乏可质子化的碱性中心，因此用质谱仪(MS)分析具有低信噪比和高检测下限的特点。因此，必须彻底优化葡聚糖样品制备的每一步，以将样品损失、污染和分析变异性降至最低。重要的是，必须彻底研究葡聚糖及其衍生对应物的性质，以便开发某些特征来加强MS分析。在此，研究并确定了引入永久电荷的有效性以阻碍多糖分析。此外，还优化并概述了糖肼形成的程序，其中使用部分析因设计(FFD)同时分析了大量的变量，以确定哪些条件影响肼形成反应的反应效率。最后，多糖的疏水标记被证明是进一步增加MS中聚糖离子丰度的一个可行的机会。
The analysis of N-linked glycans by mass spectrometry (MS) has been characterized by low signal-to-noise ratios and high limits of detection due to their hydrophilicity and lack of basic sites able to be protonated. As a result, every step in glycan sample preparation must be thoroughly optimized in order to minimize sample loss, contamination, and analytical variability. Importantly, properties of glycans and their derivatized counterparts must be thoroughly studied in order to exploit certain characteristics for enhancing MS analysis. Herein, the effectiveness of the incorporation of a permanent charge is studied and determined to hamper glycan analysis. Also, a procedure for glycan hydrazone formation is optimized and outlined where a large number of variables were simultaneously analyzed using a fractional factorial design (FFD) in order to determine which conditions affected the reaction efficiency of the hydrazone formation reaction. Finally, the hydrophobic tagging of glycans is shown to be a viable opportunity to further increase the ion abundance of glycans in MS.