Uptake and Toxicity Studies of Poly‐Acrylic Acid Functionalized Silicon Nanoparticles in Cultured Mammalian Cells
Uptake and Toxicity Studies of Poly‐Acrylic Acid Functionalized Silicon Nanoparticles in Cultured Mammalian Cells
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DOI：
10.1002/adhm.201100010
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发表时间：
2012-03
影响因子：
10
通讯作者：
Qi Wang;Y. Bao;Xiaohong Zhang;P. Coxon;U. Jayasooriya;Y. Chao
中科院分区：
文献类型：
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作者：
Qi Wang;Y. Bao;Xiaohong Zhang;P. Coxon;U. Jayasooriya;Y. Chao
合成了聚丙烯酸（PAAc）封端的硅纳米颗粒（SiNPs），并在一系列培养的哺乳动物细胞中用作同步荧光信号指示剂：HHL 5，HepG 2和3 T3-L1。已经研究了它们对人和小鼠细胞系中细胞生长和增殖的生物学效应。当通过细胞形态学、细胞增殖和活力以及DNA损伤试验进行评估时，没有证据表明暴露于PAAc封端的SiCl 4的细胞具有体外细胞毒性。通过共聚焦显微镜和流式细胞术研究了HepG 2和3 T3-L1细胞对纳米晶体的摄取，其在较高浓度下显示出明显的时间依赖性。重建的3-D共聚焦显微镜图像显示PAAc-SiNP均匀分布在整个细胞溶质中，而不是附着在外膜上。本研究为硅纳米粒子的安全应用和进一步修饰提供了基础证据，有望拓宽其作为细胞标记物在生命系统和胶束包裹药物传递系统中的应用。
Poly‐acrylic acid (PAAc) terminated silicon nanoparticles (SiNPs) have been synthesized and employed as a synchronous fluorescent signal indicator in a series of cultured mammalian cells: HHL5, HepG2 and 3T3‐L1. Their biological effects on cell growth and proliferation in both human and mouse cell lines have been studied. There was no evidence of in vitro cytotoxity in the cells exposed to PAAc terminated SiNPS when assessed by cell morphology, cell proliferation and viability, and DNA damage assays. The uptake of the nanocrystals by both HepG2 and 3T3‐L1 cells was investigated by confocal microscopy and flow cytometry, which showed a clear time‐dependence at higher concentrations. Reconstructed 3‐D confocal microscope images exhibited that the PAAc‐SiNPs were evenly distributed throughout the cytosol rather than attached to outer membrane. This study provides fundamental evidence for the safe application and further modification of silicon nanoparticles, which could broaden their application as cell markers in living systems and in micelle encapsulated drug delivery systems.