Isolation and characterization of microsatellite loci in the endangered Amur ratsnake Elaphe schrenckii (Reptilia; Squamata; Colubridae) from China

Isolation and characterization of microsatellite loci in the endangered Amur ratsnake Elaphe schrenckii (Reptilia; Squamata; Colubridae) from China
复制标题

中国濒危阿穆尔蛇 Elaphe schrenckii(爬行纲;有鳞目;游蛇科)微卫星位点的分离和表征

DOI:
10.1007/s12686-014-0166-z
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发表时间:
2014-03
影响因子:
1.1
通讯作者:
Li, Hong
Li, Hong
中科院分区:
环境科学与生态学4区
文献类型:
--
作者:
Zheng, Xiao-xiao;Zhu, Chao-nan;Sun, Shan;Li, Hong

文献摘要

相似文献

描述了濒危东北锦蛇的8个多态性微卫星座位。在31个无关的E.采自中国辽宁省。这些标记揭示了高度的遗传多样性(每个位点6-20个等位基因)和杂合性(HO范围为0.296 - 1.000,HE范围为0.469 - 0.850)。经Bonferroni校正后,除1个位点显著偏离Hardy-Weinberg平衡外,其余位点间均未发现显著的连锁不平衡。这些基因座均为首次发现,为进一步研究该群体的遗传结构、保护管理和亲子鉴定提供了依据。schrenckii。
Eight polymorphic microsatellite loci are described for the endangered Amur ratsnake, Elaphe schrenckii (Colubridae). Loci were screened in 31 unrelated individuals of E. schrenckii collected from Liaoning Province, China. These markers revealed a high degree of genetic diversity (6–20 alleles per locus) and heterozygosity (HO ranged from 0.296 to 1.000, and HE ranged from 0.469 to 0.850). No significant linkage disequilibrium between pairs of loci was found, except one of them significantly deviated from Hardy–Weinberg equilibrium after Bonferroni correction. These loci are the first time to be characterized and will be useful for population genetic structure investigation, conservation management and paternity identification study for E. schrenckii.