The melanin-concentrating hormone receptor 1, a novel target of autoantibody responses in vitiligo
The melanin-concentrating hormone receptor 1, a novel target of autoantibody responses in vitiligo
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DOI：
10.1172/jci14643
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发表时间：
2002-04-01
影响因子：
15.9
通讯作者：
Watson, PF
中科院分区：
文献类型：
--
作者：
Kemp, EH;Waterman, EA;Watson, PF
白癜风是一种常见的色素脱失性疾病，由皮肤中的黑素细胞损失引起。该病的发病机制尚不清楚，但认为与自身免疫机制有关。事实上，在一些白癜风患者中已经报道了靶向黑素细胞的自身抗体和自身反应性T淋巴细胞。本研究的目的是确定色素细胞抗原识别的自身抗体在白癜风。利用白癜风患者的IgG筛选黑素细胞cDNA噬菌体展示文库，我们确定了黑素浓集激素受体I（MCHR1）作为一种新的自身抗原与这种疾病。白癜风患者血清中的受体的免疫反应性证明通过使用放射性结合测定。在来自健康对照和来自自身免疫性疾病患者的血清中，没有一个表现出对MCHR1的免疫反应性，表明针对该受体的Ab的高疾病特异性。还显示了来自白癜风患者的IgG对MCH与其受体结合的抑制。
Vitiligo is a common depigmenting disorder resulting from the loss of melanocytes in the skin. The pathogenesis of the disease remains obscure, although autoimmune mechanisms are thought to be involved. Indeed, autoantibodies and autoreactive T lymphocytes that target melanocytes have been reported in some vitiligo patients. The objective of this study was to identify pigment cell antigens that are recognized by autoantibodies in vitiligo. Using IgG from vitiligo patients to screen a melanocyte cDNA phage-display library, we identified the melanin-concentrating hormone receptor I (MCHR1) as a novel autoantigen related to this disorder. Immunoreactivity against the receptor was demonstrated in vitiligo patient sera by using radiobinding assays. Among sera from healthy controls and from patients with autoimmune disease, none exhibited immunoreactivity to MCHR1, indicating a high disease specificity for Ab's against the receptor. Inhibition of MCH binding to its receptor by IgG from vitiligo patients was also shown.