Desiccation, thermal stress and associated mortality in Drosophila fruit flies induced by neuropeptide analogue treatment
Desiccation, thermal stress and associated mortality in Drosophila fruit flies induced by neuropeptide analogue treatment
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DOI：
10.1007/s10340-019-01100-0
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发表时间：
2019-06-01
影响因子：
4.8
通讯作者：
Davies, Shireen A.
中科院分区：
文献类型：
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作者：
Alford, Lucy;Marley, Richard;Davies, Shireen A.
铃木果蝇是世界范围内严重危害软果的害虫。随着全球对广谱农药的过度依赖，迫切需要更环保和有针对性的控制方法。一个有希望的途径是使用合成神经肽类似物作为杀虫剂来降低害虫的适应性。神经肽是调节生理和行为过程的核心，在寒冷和干燥的生存中起着至关重要的作用。在此基础上，本研究研究了生物稳定的激肽、心脏加速肽CAP2b和焦激肽（PK）类似物（后者先前已与capa受体发生串扰）对害虫苏氏夜蛾和与其密切相关的非害虫黑腹夜蛾的干燥、饥饿和冷胁迫耐受性的影响。结果表明，超家族的类似物（CAP2b和PK衍生）显著影响目标昆虫在干燥胁迫条件下的存活。然而，与对照组相比，这些肽提高了干燥胁迫下的存活率，这表明它们可能是马氏小管中capa信号通路的拮抗剂。特别值得注意的是，类似物1895 （2Abf-Suc-FGPRLa）和1902 （2Abf-Suc-FKPRLa）对铃木致病菌有影响，但对黑腹致病菌没有影响。关注原生果蝇CAP2b/PK和激肽序列的类似物开发可能会产生具有利尿作用的纯激动剂，可能会降低害虫蝇的干燥应激存活率。通过对PRXamide神经肽超家族的广泛研究，以及对有前景的类似物结构的更具体的研究，本研究将为下一代类似物的进化提供支持，并推动神经肽仿生药物的发展。
Drosophila suzukii is a serious pest of soft fruit worldwide. With the global over-dependence on broad-spectrum pesticides, a strong imperative exists for more environmentally friendly and targeted methods of control. One promising avenue involves employing synthetic neuropeptide analogues as insecticidal agents to reduce pest fitness. Neuropeptides, central to the regulation of physiological and behavioural processes, play a vital role in cold and desiccation survival. Building upon this, the current study investigated the effects of biostable kinin, the cardioacceleratory peptide CAP2b and pyrokinin (PK) analogues (the latter of which have previously displayed cross-talk with the capa receptor), on desiccation, starvation and cold stress tolerance of the pest, D. suzukii, and the closely related non-pest, D. melanogaster. Results demonstrated analogues of the superfamily (CAP2b and PK derived) significantly impacted survival of the target insect under conditions of desiccation stress. However, these peptides enhanced desiccation stress survival in relation to controls, suggesting that they may act as antagonists of the capa signalling pathway in the Malpighian tubules. Of particular note was the ability of analogues 1895 (2Abf-Suc-FGPRLa) and 1902 (2Abf-Suc-FKPRLa) to impact D. suzukii but not D. melanogaster. A focus on native Drosophila CAP2b/PK and kinin sequences in analogue development may yield pure agonists with diuretic action that may reduce desiccation stress survival in the pest flies. In highlighting the PRXamide neuropeptide superfamily more generally, and the structures of promising analogues more specifically, this research will feed the evolution of next-generation analogues and drive forward the development of neuropeptidomimetic-based agents.