Nanoindentation discriminates the elastic properties of individual human bone lamellae under dry and physiological conditions
Nanoindentation discriminates the elastic properties of individual human bone lamellae under dry and physiological conditions
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DOI：
10.1016/s8756-3282(01)00624-x
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发表时间：
2002-01-01
期刊：
影响因子：
4.1
通讯作者：
Zysset, P
中科院分区：
文献类型：
--
作者：
Hengsberger, S;Kulik, A;Zysset, P
采用原子力显微镜（AFM）和纳米压痕技术相结合，测量了人类致密骨和小梁骨组织单层的机械性能。这种组合既可以表征骨表面形貌，又可以表征深度在 100 至 600 nm 之间的骨细胞外基质 (ECM)。在干燥条件下对四个具有 400 个凹痕的骨结构单元 (BSU) 进行测试，在生理条件下在液体细胞中对四个具有 160 个凹痕的 BSU 进行测试。在骨板的光学外观和抛光骨表面的形貌之间建立了对应关系。研究了干湿条件下骨 ECM 的压痕模量和硬度与片层类型和压痕深度的函数关系。对于低深度压痕，厚片层比薄片层表现出更高的压痕模量。随着压痕深度的增加，厚片层的压痕模量和硬度显着降低，而对于薄片层，压痕深度的影响则不那么显着。这些趋势在干燥和生理条件下是相似的，并且支持厚片层和薄片层之间的成分和/或超微结构差异。 (C) 2002 年，Elsevier Science Inc. 保留所有权利。
Mechanical properties of single lamellae of human compact and trabecular bone tissue were measured with a combined atomic force microscopy (AFM) and nanoindentation technique. This combination allows for both characterization of bone surface topography and indentation of the bone extracellular matrix (ECM) with depths of between 100 and 600 nm. Four bone structural units (BSUs) were tested with 400 indents under dry conditions, and four BSUs with 160 indents were tested in a liquid cell under physiological conditions. A correspondence was established between the optical appearance of bone lamellae and the topography of the polished bone surface. The indentation modulus and hardness of bone ECM were investigated as a function of lamella type and indentation depth under wet and dry conditions. For low depth indents, thick lamellae showed a higher indentation modulus than thin lamellae. With increasing indentation depth, thick lamellae exhibited a significant decrease in indentation modulus and hardness, whereas, for thin lamellae, the effect of indentation depth was much less significant. These trends were similar for dry and physiological conditions and support compositional and/or ultrastructural differences between thick and thin lamellae. (C) 2002 by Elsevier Science Inc. All rights reserved.