Genetic organization and preferential distribution of putative pilus gene clusters in Streptococcus suis
Genetic organization and preferential distribution of putative pilus gene clusters in Streptococcus suis
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DOI：
10.1016/j.vetmic.2009.02.013
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发表时间：
2009-07-02
影响因子：
3.3
通讯作者：
Sekizaki, Tsutomu
中科院分区：
文献类型：
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作者：
Takamatsu, Daisuke;Nishino, Hiroto;Sekizaki, Tsutomu
最近使用多位点序列分型 (MLST) 对猪链球菌分离株进行的分析表明序列类型 (ST) 1 和 ST27 复合物对于动物卫生和公共卫生的重要性。在本研究中，为了调查猪链球菌菌毛相关基因是否可以用作重要克隆群体的新遗传标记，我们检查了猪链球菌中 ST 和假定的菌毛相关基因谱之间的相关性。使用测序基因组和在几个分离株中确定的序列数据进行的基因组搜索揭示了猪链球菌中至少存在四个不同的推定菌毛基因簇（srtBCD、srtE、srtF 和 srtG 簇）。根据四个簇中是否存在基因，将来自不同来源的 108 个猪链球菌分离株分为 12 个基因型（基因型 A-L）。基因型A和B分别具有srtBCD加srtF簇和srtF加srtG簇，在病猪和人的分离株中最常见，分别有29.9%和59.8%的分离株属于基因型A和B。相比之下，来自健康携带者的分离株分别只有 4.8% 和 28.6% 是基因型 A 和 B。 MLST 分析显示基因型 A 和 B 分别与 ST1 和 ST27 复合物相关。此外，srtBCD和srtG簇分别优先分配给ST1和ST27复合体成员。这些结果表明，对选定的菌毛相关基因进行分析可以作为一种简单的筛选方法来监测对猪链球菌感染重要的分离株。 (C) 2009 Elsevier B.V. 保留所有权利。
Recent analyses of Streptococcus suis isolates using multilocus sequence typing (MLST) suggested the importance of sequence type (ST) 1 and ST27 complexes for animal hygiene and public health. In this study, to investigate whether pilus-associated genes in S. suis can be used as novel genetic markers for important clonal groups, we examined the correlation between STs and putative pilus-associated gene profiles in S. suis. Genomic searches using sequenced genomes and sequence data determined in several isolates revealed the presence of at least four distinct putative pilus gene clusters in S. suis (srtBCD, srtE, srtF and srtG clusters). On the basis of the presence or absence of genes in the four clusters, 108 S. suis isolates from various origins were classified into 12 genotypes (genotypes A-L). Genotypes A and B, which possessed srtBCD plus srtF clusters and srtF plus srtG clusters, respectively, were the most common in isolates from diseased pigs and humans, and 29.9% and 59.8% of the isolates belonged to genotypes A and B, respectively. In contrast, only 4.8% and 28.6% of isolates from healthy carriers were genotypes A and B, respectively. MLST analysis showed the associations of genotypes A and B with ST1 and ST27 complexes, respectively. In addition, srtBCD and srtG clusters were preferentially distributed to ST1 and ST27 complex members, respectively. These results suggest that profiling of selected pilus-associated genes could be used as an easy screening method to monitor isolates important for S. suis infection. (C) 2009 Elsevier B.V. All rights reserved.