Deinococcus radiodurans DR1088 is a novel RecF-interacting protein that stimulates single-stranded DNA annealing
Deinococcus radiodurans DR1088 is a novel RecF-interacting protein that stimulates single-stranded DNA annealing
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Deinococcus radiodurans DR1088 是一种新型 RecF 相互作用蛋白，可刺激单链 DNA 退火。
DOI：
10.1111/mmi.13828
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发表时间：
2017-11-01
影响因子：
3.6
通讯作者：
Hua, Yuejin
中科院分区：
文献类型：
--
作者：
Cheng, Kaiying;Xu, Guangzhi;Hua, Yuejin
RecF与重组介质RecO和RecR在细菌RecFOR同源重组修复途径中是必需的。本研究在耐辐射球菌中发现了一个在耐辐射球菌-热球菌门高度保守的recF-dr1088操纵子。通过酵母双杂交和拉下实验证实了DRRecF与DR1088的互作。DR1088表现出一些类似rec的生化特性，包括单双链DNA结合活性、ssDNA结合蛋白（SSB）替代能力和ssDNA（带或不带SSB）退火活性。然而，与其他重组蛋白不同，dr1088对细胞存活至关重要。这些结果表明DR1088可能在DNA复制和DNA修复过程中发挥作用。
RecF, together with the recombination mediators RecO and RecR, is required in the RecFOR homologous recombination repair pathway in bacteria. In this study, a recF-dr1088 operon, which is highly conserved in the Deinococcus-Thermus phylum, was identified in Deinococcus radiodurans. Interaction between DRRecF and DR1088 was confirmed by yeast two-hybrid and pull-down assays. DR1088 exhibited some RecO-like biochemical properties including single/double-stranded DNA binding activity, ssDNA binding protein (SSB) replacement ability and ssDNA (with or without SSB) annealing activity. However, unlike other recombination proteins, dr1088 is essential for cell viability. These results indicate that DR1088 might play a role in DNA replication and DNA repair processes.