Convergence of bladder and colon sensory innervation occurs at the primary afferent level
Convergence of bladder and colon sensory innervation occurs at the primary afferent level
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DOI：
10.1016/j.pain.2006.09.023
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发表时间：
2007-04-01
期刊：
影响因子：
7.4
通讯作者：
Pezzone, Michael A.
中科院分区：
文献类型：
--
作者：
Christianson, Julie A.;Liang, Ruomei;Pezzone, Michael A.
二分类传入神经是指单独的背根神经节（DRG）神经元支配两个不同的结构，从而提供一种可能参与盆腔器官交叉敏化的传入收敛形式。为了确定供应Sprague-Dawley大鼠和C57Bl/6小鼠远端结肠和膀胱的二元传入管的分布，我们使用霍乱毒素亚单位B （CTB）荧光偶联物对膀胱和远端结肠传入管进行了同时逆行标记。浆膜下器官注射后灌注4-5天，取T10-S2 DRG，切片，共聚焦显微镜分析。在大鼠中，膀胱逆行标记的ctb阳性传入事件几乎是远端结肠标记的传入事件的三倍，而在小鼠中，每个器官都有相同的代表。在这两个物种中，大部分的结肠和膀胱传入神经来自腰骶神经节，其次来自胸腰神经节。在大鼠中，17%的ctb阳性神经元从两个器官逆行标记，其中11%位于TL， 6%位于LS， 0.8%位于胸（TH）神经节。在小鼠中，总ctb阳性神经元中有21%是双标记的，其中12%定位于LS， 4%定位于TH， 4%定位于TL神经节。这些发现支持骨盆传入事件的存在，它为可能的即时和维持盆腔器官交叉致敏提供了一个预先存在的神经元基质，并最终可能在盆腔疼痛疾病的重叠中发挥作用。(c) 2006国际疼痛研究协会。Elsevier B.V.版权所有。
Dichotomizing afferents are individual dorsal root ganglion (DRG) neurons that innervate two distinct structures thereby providing a form of afferent convergence that may be involved in pelvic organ cross-sensitization. To determine the distribution of dichotomizing afferents supplying the distal colon and bladder of the Sprague-Dawley rat and the C57Bl/6 mouse, we performed concurrent retrograde labeling of urinary bladder and distal colon afferents using cholera toxin Subunit B (CTB) fluorescent conjugates. Animals were perfused 4-5 days after sub-serosal organ injections, and the T10-S2 DRG were removed, sectioned, and analyzed using confocal microscopy. In the rat, CTB-positive afferents retrogradely labeled from the bladder were nearly three times more numerous than those labeled from the distal colon, while in the mouse, each organ was equally represented. In both species, the majority of colon and bladder afferents projected from lumbosacral (LS) ganglia and secondarily from thoracolumbar (TL) ganglia. In the rat, 17% of the total CTB-positive neurons were retrogradely labeled from both organs with 11% localized in TL, 6% in LS, and 0.8% in thoracic (TH) ganglia. In the mouse, 21% of the total CTB-positive neurons were dually-labeled with 12% localized in LS, 4% in TH, and 4% in TL ganglia. These findings support the existence of dichotomizing pelvic afferents, which provide a preexisting neuronal substrate for possible immediate and maintained pelvic organ cross-sensitization and ultimately may play a role in the overlap of pelvic pain disorders. (c) 2006 International Association for the Study of Pain. Published by Elsevier B.V. All rights reserved.