Genetic toxicology of the photosensitization of Chinese hamster cells by phthalocyanines.
Genetic toxicology of the photosensitization of Chinese hamster cells by phthalocyanines.
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酞菁对中国仓鼠细胞光敏作用的遗传毒理学。
DOI：
10.1111/j.1751-1097.1987.tb05368.x
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发表时间：
1987
影响因子：
3.3
通讯作者：
Elkind,MM
中科院分区：
文献类型：
--
作者：
Ben-Hur,E;Fujihara,T;Suzuki,F;Elkind,MM
氯铝酞菁（chloroaluminium phthalocyanine，CAPC）是近年来发现的一种具有光敏性的细胞杀伤和体内肿瘤杀伤作用的化合物，由于其在光动力学治疗癌症方面具有潜在的应用价值，因此对其作为基因毒性剂的特性进行了评价。应用碱性洗脱技术研究DNA完整性，发现CAPC在白色荧光灯照射后可使中国仓鼠细胞DNA发生单链断裂。在等细胞毒性剂量下，CAPC光敏化产生的DNA链断裂数量比X射线照射诱导的DNA链断裂数量低约三倍。在暴露于CAPC+荧光灯后在生长培养基中孵育期间，细胞以与X射线照射后观察到的相似的速率重新连接DNA链断裂。对6-硫代鸟嘌呤（6-TG '）或哇巴因（OUA'）的抗性被用作致突变潜力的终点。在导致约90%细胞杀伤的处理后，存在轻微的致突变效应，即频率比背景或自发突变增加约40%。然而，这种增强没有统计学意义。综上所述，加上早期观察到的细胞对CAPC +光的敏感性在整个细胞周期中没有变化，导致DNA损伤在细胞杀伤中不起主要作用，并且这种处理的致突变潜力很小。
Chloroaluminum phthalocyanine (CAPC) was recently shown to photosensitize cell killing in culture and tumor destructionin vivo.Because this compound is potentially useful in the photodynamic therapy of cancer, its properties as a genotoxic agent were evaluated. Applying the technique of alkaline elution to study DNA integrity, it was found that CAPC could produce single‐strand breaks in the DNA of Chinese hamster cells after exposure to white fluorescent light. At equicytotoxic doses, the number of DNA strand breaks produced by CAPC photosensitization was about three times lower than that induced by X‐irradiation. During incubation in growth medium after exposure to CAPC‐plus‐fluorescent light, cells rejoined DNA strand breaks at a rate similar to that observed after X‐irradiation. Resistance to 6‐thioguanine (6‐TG') or to ouabain (OUA') were used as end points of mutagenic potential. Following a treatment that caused ‐90% cell killing, there was a slight mutagenic effect, i.e. the frequencies were increased by ‐40% above the background or spontaneous mutations. However, this enhancement was not statistically significant. Taken together, the foregoing, plus an earlier observation that there is no variation in the sensitivity of cells to CAPC + light through the cell cycle, lead to the inferences that DNA damage does not play a major role in cell killing and that the mutagenic potential of this treatment is small.