PTGER2 activation induces PTGS-2 and growth factor gene expression in endometrial epithelial cells of cattle
PTGER2 activation induces PTGS-2 and growth factor gene expression in endometrial epithelial cells of cattle
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PTGER2 激活诱导牛子宫内膜上皮细胞中 PTGS-2 和生长因子基因的表达。
DOI：
10.1016/j.anireprosci.2017.10.005
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发表时间：
2017-12-01
影响因子：
2.2
通讯作者：
Cao, Jinshan
中科院分区：
文献类型：
--
作者：
Gao, Long;Liu, Bo;Cao, Jinshan
前列腺素E2受体2（PTGER 2）存在于子宫内膜中，其基因表达伴随着子宫内膜的生长，然而，尚不清楚牛中是否存在通过PTGER 2激活促进的生长因子基因表达刺激的子宫内膜修复。本研究的目的是探讨PTGER 2激活是否可以通过激活PKA和ERK信号通路诱导牛子宫内膜上皮细胞中的前列腺素-内过氧化物合酶-2（PTGS-2）和生长因子基因的表达。结果表明，PTGER 2激动剂布他前列素诱导cAMP/PKA和ERK活化，并上调PTGS-2、VEGF、CTGF、TGF-131和IL-8基因表达。PTGER 2拮抗剂AH 6809治疗后，这些激活较少。数据表明，PTGS-2基因表达由PTGER 2通过PKA和ERK途径激活诱导。此外，PTGER 2激活促进子宫内膜上皮细胞中几种生长因子基因的表达。这一发现的一个潜在含义是，牛子宫内膜中PTGER 2的活化可通过刺激VEGF、CTGF、TGF-131和IL-8基因表达来诱导子宫内膜修复。
The prostaglandin E2 receptor 2 (PTGER2) is present in the endometrium and its gene expression is accompanied with endometrial growth, however, it is unknown whether there is endometrial repair through stimulation of growth factor gene expression that is promoted by PTGER2 activation in cattle. The aim of this study was to investigate whether PTGER2 activation can induce prostaglandin-endoperoxide synthase-2 (PTGS-2) and growth factor gene expression by activating PKA and ERK signaling pathways in endometrial epithelial cells of cattle. Results demonstrated that the PTGER2 agonist, butaprost, induced cAMP/PKA and ERK activation and up regulated PTGS-2, VEGF, CTGF, TGF-131 and IL-8 gene expression. These activations were less after PTGER2 antagonist, AH6809, treatment. Data suggested that PTGS-2 gene expression was induced by PTGER2 activation through the PKA and ERK pathways. Furthermore, PTGER2 activation promoted several growth factor gene expressions in endometrial epithelial cells. One potential implication of this fmding is that PTGER2 activation in the endometrium of cattle could induce endometrial repair by stimulating VEGF, CTGF, TGF-131 and IL-8 gene expression.