Evaluation of different methods to detect methicillin resistance in small-colony variants of Staphylococcus aureus
Evaluation of different methods to detect methicillin resistance in small-colony variants of Staphylococcus aureus
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DOI：
10.1128/jcm.42.3.1277-1279.2004
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发表时间：
2004-03-01
影响因子：
9.4
通讯作者：
von Eiff, C
中科院分区：
文献类型：
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作者：
Kipp, F;Becker, K;von Eiff, C
为了评估不同方法检测金黄色葡萄球菌小菌落变体 (SCV) 甲氧西林耐药性的能力，本研究使用了 11 种具有 SCV 表型的不同耐甲氧西林金黄色葡萄球菌 (MRSA) 克隆。 SCV 的缓慢生长通常使得纸片扩散或自动化方法的测试无效。只有通过 PCR 检测 mecA 基因和使用较高菌落数的 MRSA-Screen 乳胶凝集试验才被证明是快速检测这些患者中甲氧西林耐药性的可靠方法。
To evaluate different methods for their abilities to detect methicillin resistance in small-colony variants (SCVs) of Staphylococcus aureus, 11 different methicillin-resistant S. aureus (MRSA) clones with the SCV phenotype were used in this study. The slow growth of SCVs often makes testing by disk diffusion or by automated methods invalid. Only detection of the mecA gene by PCR and the MRSA-Screen latex agglutination test using a higher colony number were shown to be reliable methods to rapidly detect methicillin resistance in these