The microheterogeneity components of orosomucoid and the dissociation constants and mobilities of concanavalin A/corosomucoid complexes in crossed affinoimmunoelectrophoresis with free concanavalin A
The microheterogeneity components of orosomucoid and the dissociation constants and mobilities of concanavalin A/corosomucoid complexes in crossed affinoimmunoelectrophoresis with free concanavalin A
复制标题
口腔类粘蛋白的微观异质性成分以及伴刀豆球蛋白 A/科罗类粘蛋白复合物在与游离伴刀豆球蛋白 A 交叉亲和免疫电泳中的解离常数和迁移率
DOI：
10.1002/elps.1150050403
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发表时间：
1984
期刊：
影响因子：
2.9
通讯作者：
Thorkild C. Bøg‐Hansenm
中科院分区：
文献类型：
--
作者：
J. Hansen;A. Lihme;Thorkild C. Bøg‐Hansenm
在交叉亲和免疫电泳与游离刀豆球蛋白A（刀豆球蛋白A）的第一维，四orosomucoid成分通常被发现在人血清中。在本研究中，定义了第一维凝胶中Con A浓度的最佳范围。最延迟的，类粘蛋白的第四组分被证明是亲和沉淀物中Con A结合分子截留的结果。的亲和力的第二，弱延迟，组件和第三，强烈延迟，组件的Con A结合位点被发现是相同的。发现orosomucoid/Con A复合物的第一维电泳中的迁移率彼此显著不同。与orosomucoid的第一组分的非阻滞性流动性相比，第二组分的流动性为40%，第三组分的流动性为10%。在触角糖基化位点的orosomucoid糖结构的差异建议作为这些意见的解释。
In crossed affinoimmunoelectrophoresis with free concanavalin A (Con A) in the first dimension, four orosomucoid components are normally found in human serum. In this study an optimal range of Con A concentration in the first‐dimension gel was defined. The most retarded, fourth component of orosomucoid was shown to be a result of entrapment of Con A‐binding molecules in the affinity precipitate. The affinities of the second, weakly retarded, component and of the third, strongly retarded, component to a Con A binding site were found to be identical. The mobilities in the first‐dimension electrophoresis of the orosomucoid/Con A complexes were found to be significantly different from each other. Compared to the mobility of the nonretarded, first, component of orosomucoid, the second component had a mobility of 40% and the third component had a mobility of 10%. Differences in the antennary carbohydrate structures on the glycosylation sites of orosomucoid are suggested as an explanation for these observations.