Eradication of a mature methicillin-resistant Staphylococcus aureus (MRSA) biofilm from acrylic surfaces.
Eradication of a mature methicillin-resistant Staphylococcus aureus (MRSA) biofilm from acrylic surfaces.
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DOI：
10.1590/0103-6440201302289
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发表时间：
2013-09
影响因子：
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通讯作者：
Karen Tereza Altieri;P. Sanitá;A. Machado;E. T. Giampaolo;A. C. Pavarina;J. Jorge;C. Vergani
中科院分区：
文献类型：
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作者：
Karen Tereza Altieri;P. Sanitá;A. Machado;E. T. Giampaolo;A. C. Pavarina;J. Jorge;C. Vergani
耐甲氧西林金黄色葡萄球菌（MRSA）可以在不同的表面生长，包括口腔粘膜和义齿表面。这种生物膜可以释放到口腔液中并被吸出，引起全身感染，如吸入性肺炎。本研究评价了两种消毒剂溶液和微波辐照对MRSA生物膜污染的丙烯酸样本的消毒效果。制备36个丙烯酸样本，灭菌并用MRSA（107 cfu/mL）污染。孵育（37 °C/48 h）后，将标本分为4组：不消毒（阳性对照）; 1%次氯酸钠浸泡10 min; 2%葡萄糖酸氯己定浸泡10 min; 650 W微波照射3 min。XTT还原法检测细胞活力。培养48 h后，阳性对照组的所有标本均显示生物膜形成。对照样本的平均吸光度值为1.58（492 nm处的OD）。在消毒方法后，在标本上未观察到生物膜形成的证据。用1%次氯酸钠和2%葡萄糖酸氯己定浸泡消毒并微波照射，导致MRSA生物膜代谢100%减少。使用化学溶液和微波辐射被证明是有效的消除成熟的MRSA生物膜上的丙烯酸树脂标本。
Methicillin-resistant Staphylococcus aureus (MRSA) can grow as structured biofilm in different surfaces, including oral mucosa and denture surfaces. Such biofilms can be released into the oral fluids and aspirated, causing systemic infections such as aspiration pneumonia. This study evaluated the efficacy of two disinfectant solutions and microwave irradiation in disinfecting acrylic specimens contaminated with MRSA biofilm. Thirty-six acrylic specimens were made, sterilized and contaminated with MRSA (107 cfu/mL). After incubation (37 °C/48 h), the specimens were divided into 4 groups: not disinfected (positive control); soaking in 1% sodium hypochlorite for 10 min; soaking in 2% chlorhexidine gluconate for 10 min; and irradiating by microwave for 3 min at 650 W. The viability of cells was evaluated by XTT reduction method. All specimens from the positive control group showed biofilm formation after 48 h incubation. The mean absorbance value of the control specimens was 1.58 (OD at 492 nm). No evidence of biofilm formation was observed on specimens after the disinfection methods. Disinfection by soaking in 1% sodium hypochlorite and 2% chlorhexidine gluconate and irradiating by microwaves resulted in 100% reduction of MRSA biofilm metabolism. The use of chemical solutions and microwave irradiation was shown to be effective for eradicating mature MRSA biofilms on acrylic resin specimens.