Autonomous control of phosphatidylinositol turnover by histamine and acetylcholine receptors in the N1E-115 neuron-like cell line.
Autonomous control of phosphatidylinositol turnover by histamine and acetylcholine receptors in the N1E-115 neuron-like cell line.
复制标题
N1E-115 神经元样细胞系中组胺和乙酰胆碱受体自主控制磷脂酰肌醇周转。
DOI：
10.1016/0304-3940(86)90161-8
复制
发表时间：
1986
影响因子：
2.5
通讯作者：
Klein,WL
中科院分区：
文献类型：
--
作者：
Large,TH;Lambert,MP;Cohen,NM;Klein,WL
组胺被发现刺激营业额的磷脂酰肌醇（PI）在培养的神经元样NE-115细胞。通过增加[3 H]肌醇磷酸的产生（分解）和加速32 P掺入PI（再合成）来测量周转率。数据与多磷酸肌醇水解是受体刺激PI周转的初始事件一致。组胺脱敏反应在10分钟内。组胺和乙酰胆碱受体系统进行了测试，可能的相互作用：PI营业额在响应双重刺激约等于个人的反应，而之前脱敏的乙酰胆碱受体系统没有影响随后的刺激组胺受体系统的总和。这些结果是一致的假设，负责PI营业额的乙酰胆碱和组胺受体系统的组件是自主组织和调节。
Histamine was found to stimulate the turnover of phosphatidylinositol (PI) in cultures of neuron-like NE-115 cells. Turnover was measured by increased production of [3H]inositol phosphates (breakdown) and by accelerated incorporation of32P into PI (resynthesis). Data were consistent with hydrolysis of polyphosphoinositides being the inital event in receptor-stimulated PI turnover. This response to histamine desensitized within 10 min. Receptor systems for histamine and acetylcholine were tested for possible interactions: PI turnover in response to dual stimulation was approximately equal to the sum of the individual responses while prior desensitization of the acetylcholine receptor system had no effect on subsequent stimulation of the histamine receptor system. These results are consistent with the hypothesis that components of acetylcholine and histamine receptor systems responsible for PI turnover are autonomously organized and regulated.