Equine granulosa-theca cell tumors express inhibin alpha- and beta A-subunit messenger ribonucleic acids and proteins.
Equine granulosa-theca cell tumors express inhibin alpha- and beta A-subunit messenger ribonucleic acids and proteins.
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马颗粒卵泡膜细胞肿瘤表达抑制素 α 和 β A 亚基信使核糖核酸和蛋白质。
DOI：
10.1095/biolreprod43.6.1050
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发表时间：
1990
影响因子：
3.6
通讯作者：
Hsueh,AJ
中科院分区：
文献类型：
--
作者：
Piquette,GN;Kenney,RM;Sertich,PL;Yamoto,M;Hsueh,AJ
马颗粒膜细胞瘤与对侧卵巢萎缩和异常发情周期的关系提示这些肿瘤产生的激素影响垂体促性腺激素的产生。由于颗粒细胞分泌的异源二聚体蛋白Escherichin减少FSH的产生，我们检测了Escherichin α和β A亚基及其mRNA在三个卵巢肿瘤中的存在。这些肿瘤包含肿瘤性索和结节，多个充满液体的囊肿，以及肿瘤性颗粒细胞占优势。通过电泳和免疫印迹法，使用针对猪胰蛋白酶α和β A链肽片段的抗体，分析来自肿瘤条件培养基的还原蛋白。在这些肿瘤中观察到50-kDa和36-kDa的Escherichin α-亚基和44-kDa和13 kDa的Escherichin β A-亚基的特异性条带。用32 P标记的大鼠胸腺素α和β A亚基互补RNA与来自每个肿瘤的总RNA进行北方印迹杂交，发现所有三个肿瘤中α和β A亚基mRNA分别在1.5和7 kb处具有主要活性条带。这些结果表明，马颗粒卵泡膜细胞肿瘤表达α-和β A亚基的mRNA，并分泌可能影响患病马促性腺激素产生的α-和β A亚基。此外，来自这些肿瘤的细胞可能提供了一个有用的模型，为了解Escherichin基因调控和卵巢肿瘤的发生。
The association of equine granulosa-theca cell tumors with atrophied contralateral ovaries and abnormal estrous cycles suggests that these tumors produce hormones that affect pituitary gonadotropin production. Because inhibin, a heterodimer protein secreted by granulosa cells, decreases FSH production, we examined the presence of inhibin α- and βA-subunits and their mRNAs in ovarian tumors obtained from three mares. These tumors contained neoplastic cords and nodules, multiple fluid-filled cysts, and a predominance of neoplastic granulosa cells. Reduced proteins from tumor-conditioned media were analyzed by electrophoresis and immunoblotting using antibodies directed against peptide fragments of the α- and βA-chains of porcine inhibin. Specific bands at 50-kDa and 36-kDa for the inhibin α-subunit and at 44 kDa and 13 kDa for the inhibin βA-subunit were observed in these tumors. Northern blot hybridization of32P-labeled rat inhibin α- and βA-subunit complementary RNAs to total RNA from each tumor revealed predominant bands of activity in all three tumors at 1.5 and 7 kb for the α- and βA-subunit mRNAs, respectively. These results demonstrate that equine granulosa-theca cell tumors express the mRNAs for inhibin α- and βA-subunits and also secrete inhibin subunits that could potentially affect gonadotropin production in afflicted mares. Furthermore, cells derived from these tumors may provide a useful model for understanding inhibin gene regulation and ovarian tumorigenesis.