Identification of anti-HIV macrocyclic daphnane orthoesters from Wikstroemia ligustrina by LC-MS analysis and phytochemical investigation
Identification of anti-HIV macrocyclic daphnane orthoesters from Wikstroemia ligustrina by LC-MS analysis and phytochemical investigation
复制标题
DOI：
10.1007/s11418-021-01551-9
复制
发表时间：
2021-07-21
影响因子：
3.3
通讯作者：
Li, Wei
中科院分区：
文献类型：
--
作者：
Otsuki, Kouharu;Zhang, Mi;Li, Wei
大环瑞香原酸酯（MDO）引起了人们对基于“休克和杀伤”策略的治疗艾滋病毒感染的药物发现的重大研究兴趣。在本研究中，通过 LC-MS 分析和植物化学研究对 Wikstroemia 女贞（瑞香科）进行了首次化学研究。通过 LC-MS 分析检测到九种瑞香二萜 (1-9)，包括七种 MDO。进一步的植物化学研究导致了五种具有有效抗 HIV 活性的瑞香（1、2、5、8 和 9）的分离和结构阐明。以分离的 MDO (1) 作为模型化合物，还阐明了 MS/MS 裂解途径。
Macrocyclic daphnane orthoesters (MDOs) have attracted significant research interest for the drug discovery to cure HIV infection based on the "Shock and Kill" strategy. In the present study, the first chemical study on Wikstroemia ligustrina (Thymelaeaceae) was carried out by LC-MS analysis and phytochemical investigation. Nine daphnane diterpenoids (1-9) including seven MDOs were detected by LC-MS analysis. Further phytochemical investigation resulted in the isolation and structural elucidation of five daphnanes (1, 2, 5, 8, and 9) with potent anti-HIV activity. Taking the isolated MDO (1) as a model compound, the MS/MS fragmentation pathway was also elucidated.