The application of epitope mapping in the development of a new serological test for Helicobacter pylori infection
The application of epitope mapping in the development of a new serological test for Helicobacter pylori infection
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DOI：
10.1016/0022-1759(96)00071-3
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发表时间：
1996-07-17
影响因子：
2.2
通讯作者：
AlDughaym, A
中科院分区：
文献类型：
--
作者：
Burnie, JP;AlDughaym, A
对幽门螺杆菌尿素酶A和尿素酶B基因的氨基酸序列进行了表位定位。这确定了15个表位，其中5个是最具免疫优势的。它们是LTPKELD(Ure A)、FISP、QIPTAF、EVGKVA和SIP(Ure B)。用代表LTPKELD的多肽1和代表EVGKVA的多肽2建立检测幽门螺杆菌感染特异性抗体的ELISA方法。多肽1反应性IgM的敏感性、特异性和效应值分别为31.6%、92.8%和52.5%，多肽1免疫球蛋白的敏感性、特异性和效应值分别为52.6%、35.7%和45.4%。多肽2免疫球蛋白M的对应值分别为31.6%、100%和60.6%，多肽2免疫球蛋白分别为63.2%、71.4%和66.6%。当两种试验结合在一起时，任何一种肽的阳性都被算作总体阳性，IgM的数字分别为52.6%、92.8%和69.6%。因此，表位图描绘了在幽门螺杆菌活动性感染中针对其产生特异性IgM的多肽。
Epitope mapping was applied to the derived amino acid sequences of the urease A and urease B genes of Helicobacter pylori. This identified 15 epitopes of which five were the most immunodominant. These were LTPKELD (Ure A), FISP, QIPTAF, EVGKVA and SIP (Ure B). Peptide 1 representing LTPKELD and peptide 2 representing EVGKVA were used to develop ELISA procedures for detecting antibody specific to H. pylori infection. The sensitivity, specificity and efficiency values for peptide 1 reactive IgM were 31.6, 92.8 and 52.5% and for peptide 1 IgG were 52.6, 35.7 and 45.4%. The corresponding values for peptide 2 IgM were 31.6, 100 and 60.6% and for peptide 2 Ige were 63.2, 71.4 and 66.6% respectively. When the tests were combined so that a positive for either peptide was counted as a positive overall the figures for IgM were 52.6, 92.8 and 69.6%. Thus epitope mapping delineated peptides against which specific IgM was produced in active H. pylori infection.