High-Resolution "Fleezers": Dual-Trap Optical Tweezers Combined with Single-Molecule Fluorescence Detection.
High-Resolution "Fleezers": Dual-Trap Optical Tweezers Combined with Single-Molecule Fluorescence Detection.
复制标题
高分辨率“Fleezers”：双阱光镊与单分子荧光检测相结合。
作者：
Kevin D. Whitley;M. Comstock;Y. Chemla
Recent advances in optical tweezers have greatly expanded their measurement capabilities. A new generation of hybrid instrument that combines nanomechanical manipulation with fluorescence detection-fluorescence optical tweezers, or "fleezers"-is providing a powerful approach to study complex macromolecular dynamics. Here, we describe a combined high-resolution optical trap/confocal fluorescence microscope that can simultaneously detect sub-nanometer displacements, sub-piconewton forces, and single-molecule fluorescence signals. The primary technical challenge to these hybrid instruments is how to combine both measurement modalities without sacrificing the sensitivity of either one. We present general design principles to overcome this challenge and provide detailed, step-by-step instructions to implement them in the construction and alignment of the instrument. Lastly, we present a set of protocols to perform a simple, proof-of-principle experiment that highlights the instrument capabilities.
登录
查看更多内容
DOI：
--
发表时间：
2015
期刊：
--
影响因子：
--
作者：
S. Suksombat
通讯作者：
S. Suksombat
影响因子：
12.4
作者：
Woodside MT;Block SM
通讯作者：
Block SM
DOI：
10.1007/978-1-4939-0329-0_10
发表时间：
2014
期刊：
Methods in molecular biology (Clifton, N.J.)
影响因子：
--
作者：
Nicholas, Matthew P;Rao, Lu;Gennerich, Arne
通讯作者：
Gennerich, Arne
DOI：
10.1073/pnas.0511048103
发表时间：
2006-04-18
影响因子：
11.1
作者：
Woodside, MT;Behnke-Parks, WM;Block, SM
通讯作者：
Block, SM