IDENTIFICATION AND STRUCTURE OF 4 YEAST GENES (SLY) THAT ARE ABLE TO SUPPRESS THE FUNCTIONAL LOSS OF YPT1, A MEMBER OF THE RAS SUPERFAMILY
IDENTIFICATION AND STRUCTURE OF 4 YEAST GENES (SLY) THAT ARE ABLE TO SUPPRESS THE FUNCTIONAL LOSS OF YPT1, A MEMBER OF THE RAS SUPERFAMILY
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DOI：
10.1128/mcb.11.2.872
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发表时间：
1991-02-01
影响因子：
5.3
通讯作者：
SCHMITT, HD
中科院分区：
文献类型：
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作者：
DASCHER, C;OSSIG, R;SCHMITT, HD
在酿酒酵母中，GTP结合的Ypt1蛋白(Ypt1p)是内质网向高尔基体转运所必需的。通过利用GAL10-YPT1融合来调节YPT1的表达，分离到了允许YPT1独立生长的三个多拷贝抑制子SLY2、SLY12和SLY41，以及一个单拷贝抑制子SLY1-20。野生型Sly1p是亲水性的，是细胞存活所必需的，与Sly1-20p只有一个氨基酸不同。SLY2和SLY12编码的蛋白具有类似于突触短蛋白的疏水尾巴，是高等真核生物中突触小泡的完整膜蛋白。Sly41P是疏水性的，与叶绿体磷酸转运体具有序列相似性。SLY12而不是SLY41是一个必需基因。SLY2缺失突变体对冷、热敏感。SLY基因产物可能包含蛋白质运输机制的元件。
In Saccharomyces cervisiae, the GTP-binding Ypt1 protein (Ypt1p) is essential for endoplasmic reticulum-to-Golgi protein transport. By exploiting a GAL10-YPT1 fusion to regulate YPT1 expression, three multicopy suppressors, SLY2, SLY12, and SLY41, and a single-copy suppressor, SLY1-20, that allowed YPT1-independent growth were isolated. Wild-type Sly1p is hydrophilic, is essential for cell viability, and differs from Sly1-20p by a single amino acid. SLY2 and SLY12 encode proteins with hydrophobic tails similar to synaptobrevins, integral membrane proteins of synaptic vesicles in higher eucaryotes. Sly41p is hydrophobic and exhibits sequence similarities with the chloroplast phosphate translocator. SLY12 but not SLY41 is an essential gene. The SLY2 null mutant is cold and heat sensitive. The SLY gene products may comprise elements of the protein transport machinery.