Free radical scavenging and antiacetylcholinesterase activities of Origanum majorana L. essential oil
Free radical scavenging and antiacetylcholinesterase activities of Origanum majorana L. essential oil
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DOI：
10.1177/0960327110391686
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发表时间：
2011-10-01
影响因子：
2.8
通讯作者：
Nawwar, G. A. M.
中科院分区：
文献类型：
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作者：
Mossa, A. T. H.;Nawwar, G. A. M.
本研究采用气相色谱-质谱仪(GC-MS)对牛至挥发油(EO)进行了分析，并对其清除自由基和抗胆碱酯酶活性进行了评价。GC-MS分析表明，主要成分为4-松油醇(29.97%)、γ-松油烯(15.40%)、反式-松油烯(10.93%)、α-松油烯(6.86%)、3-环己烯-1-甲醛、α，A4-三甲基-(S)-(CAS)(6.54%)和莎草烯(3.91%)。牛至的IC50值分别为58.67、67.11、91.25、78.67和68.75mU g/mL，对2，2‘-二苯基苦基肼(DPPH中心点)、羟基自由基、过氧化氢、还原能力和脂质过氧化的IC50值分别为23.95、44.97、51.30、42.22和52.72mU/m L。有趣的是，还具有胆碱酯酶抑制活性，其IC50值为36.40微克/毫升。我们可以得出结论，马郁兰EO具有作为一种天然抗氧化剂和抗AChE的巨大潜力。
In the present study, Origanum majorana L. essential oil (EO) was analyzed by gas chromatography-mass spectrometry (GC-MS) and evaluated for free radical scavenging and anticholinesterase activities. GC-MS analysis revealed the presence of 4-terpineol (29.97%), gamma-terpinene (15.40%), trans-sabinene hydrate (10.93), alpha-terpinene (6.86%), 3-cycolohexene-I-1 methanal,a,a4-trimethyl-,(S)-(CAS) (6.54%), and sabinene (3.91%) as main constituents. Origanum majorana L. EO exhibited concentration-dependent inhibitory effects on 2,2'-diphenylpicrylhydrazyl (DPPH center dot), hydroxyl radical, hydrogen peroxide, reducing power, and lipid peroxidation with IC50 values of 58.67, 67.11, 91.25, 78.67, and 68.75 mu g/mL, respectively; while the IC50 values for the standard trolox were noted to be 23.95, 44.97, 51.30, 42.22, and 52.72 mu g/mL, respectively. Interestingly, cholinesterase inhibitory activity was also found with IC50 values of 36.40 mu g/mL. We can conclude that the marjoram EO has a significant potential to be used as a natural antioxidant and anti-AChE.