Immunohistochemical Examination on the Distribution of Cells Expressed Lymphatic Endothelial Marker Podoplanin and LYVE-1 in the Mouse Tongue Tissue
Immunohistochemical Examination on the Distribution of Cells Expressed Lymphatic Endothelial Marker Podoplanin and LYVE-1 in the Mouse Tongue Tissue
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DOI：
10.1267/ahc.10008
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发表时间：
2010-01-01
影响因子：
2.4
通讯作者：
Sawa, Yoshihiko
中科院分区：
文献类型：
--
作者：
Noda, Yuya;Amano, Ikuko;Sawa, Yoshihiko
舌疾病的临床研究需要对舌淋巴网络和淋巴标记阳性细胞进行详细的研究。最近，有报道称，一些组织细胞和白细胞表达淋巴标志物LYVE-1和podoplanin。本研究旨在阐明LYVE-1和podoplanin表达细胞的舌分布。在小鼠舌中，podoplanin在神经鞘、舌腺肌上皮细胞和淋巴管中表达。LYVE-1在巨噬细胞标志物Mac-1阳性细胞以及淋巴管中表达，而因子VIII仅在血液内皮细胞中检测到。在血管平滑肌和肌上皮细胞中检测到α-SMA。因此，鉴定舌腺中的淋巴管、LYVE-1和因子VIII的组合或LYVE-1和Mac-1是有用的，因为肌上皮细胞表达podoplanin和α-SMA。淋巴管上因子VIII的免疫染色被LYVE-1或podoplanin的免疫染色所掩盖，因为淋巴管表达因子VIII的程度远低于血管。因此，除了唾液腺之外，podoplanin和a-SMA或因子VIII的组合可用于鉴定淋巴管和具有平滑肌或毛细血管的血管。
The clinical study for lingual disease requires the detailed investigation of the lingual lymphatic network and lymphatic marker-positive cells. Recently, it has been reported that several tissue cells and leukocytes express lymphatic markers, LYVE-1 and podoplanin. This study was aimed to clarify the lingual distribution of cells expressing LYVE-1 and podoplanin. In the mouse tongue, podoplanin is expressed in nerve sheaths, lingual gland myoepithelial cells, and lymphatic vessels. LYVE-1 is expressed in the macrophage marker Mac-1-positive cells as well as lymphatic vessels, while factor-VIII was detected in only blood endothelial cells. alpha-SMA was detected in vascular smooth muscle and myoepithelial cells. Therefore, identification of lymphatic vessels in lingual glands, the combination of LYVE-1 and factor-VIII, or LYVE-1 and Mac-1 is useful because myoepithelial cells express podoplanin and alpha-SMA. The immunostaining of factor-VIII on lymphatic vessels was masked by the immunostaining to LYVE-1 or podoplanin because lymphatic vessels express factor-VIII to a far lesser extent than blood vessels. Therefore, except for the salivary glands, the combination of podoplanin and a-SMA, or factor-VIII is useful to identify lymphatic vessels and blood vessels with smooth muscle, or blood capillaries.