Cloning and In Situ Expression Studies of the Hydrogenobaculum Arsenite Oxidase Genes
Cloning and In Situ Expression Studies of the Hydrogenobaculum Arsenite Oxidase Genes
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DOI：
10.1128/aem.00336-09
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发表时间：
2009-05-15
影响因子：
4.4
通讯作者：
McDermott, Timothy R.
中科院分区：
文献类型：
--
作者：
Clingenpeel, Scott R.;D'Imperio, Seth;McDermott, Timothy R.
新的亚砷酸盐[As（III）]氧化酶结构基因（aoxAB）从分离自酸性地热泉的产氢杆菌中克隆。逆转录PCR表明整个流出通道的表达，和aoxB cDNA克隆表现出相对于在春天的物理化学梯度的分布模式。微电极分析提供了证据的定量As（III）的转化内的微生物垫。
Novel arsenite [As(III)] oxidase structural genes (aoxAB) were cloned from Hydrogenobaculum bacteria isolated from an acidic geothermal spring. Reverse transcriptase PCR demonstrated expression throughout the outflow channel, and the aoxB cDNA clones exhibited distribution patterns relative to the physicochemical gradients in the spring. Microelectrode analyses provided evidence of quantitative As(III) transformation within the microbial mat.