Highly Stable and Multiemissive Silver Nanoclusters Synthesized in Situ in a DNA Hydrogel and Their Application for Hydroxyl Radical Sensing
Highly Stable and Multiemissive Silver Nanoclusters Synthesized in Situ in a DNA Hydrogel and Their Application for Hydroxyl Radical Sensing
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DNA 水凝胶中原位合成的高稳定多发射银纳米团簇及其在羟基自由基传感中的应用
DOI：
10.1021/acsami.8b09152
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发表时间：
2018-08-08
影响因子：
9.5
通讯作者：
Tian, Leilei
中科院分区：
文献类型：
--
作者：
Li, Jing;Yu, Jiantao;Tian, Leilei
寡核苷酸稳定的银纳米团簇（AgNCs）在生物成像和生物/化学传感方面具有广阔的应用前景。然而，其不理想的光稳定性限制了其实际应用。在这项工作中，荧光AgNC原位合成的DNA水凝胶中，由交联的酶促扩增的聚合物DNA与胞嘧啶丰富的序列在Agt的存在下，所得到的AgNC的荧光性质进行了优化，通过合理设计的DNA序列，以覆盖具有可比的绿色和红色发射的宽光谱。在DNA水凝胶的保护下，AgNCs在周围氧气环境中显示出显着改善的光稳定性，以及即使在高浓度下也具有低细胞毒性。因此，这些性质表明，滚环扩增稳定的AgNC是一种有前途的可能的荧光探针，用于检测活细胞中的活性氧/氮物质（ROS/RNS），因为红色发光物质易受氧化，从而转化为绿色发光物质。结果表明，所制备的AgNCs是一种灵敏、特异的细胞成像和ROS/RNS水平监测探针，拓宽了AgNCs的应用范围，为相关生物学研究提供了新的工具。
Oligonucleotide-stabilized silver nanoclusters (AgNCs) show promising applications in bioimaging and bio-/chemo-sensing. However, their unsatisfactory photostability limits their practical applications. In this work, fluorescent AgNCs were synthesized in situ in a DNA hydrogel, consisting of cross-linked enzymatically amplified polymeric DNAs with cytosine-rich sequences in the presence of Agt The fluorescence property of the resultant AgNCs was optimized by a rational design of the DNA sequences to cover a broad spectrum with comparable green and red emissions. Under the protection of the DNA hydrogel, the AgNCs showed significantly improved photostability in an ambient oxygen environment, as well as low cytotoxicity even at a high concentration. Therefore, these properties show the rolling-circle-amplification stabilized AgNCs to be a promising possible fluorescent probe for the detection of reactive oxygen/nitrogen species (ROS/RNS) in live cells because red-emitting species are susceptible to oxidation and consequently convert to green-emitting species. Finally, the as-prepared AgNCs were demonstrated to be a sensitive and specific probe for cellular imaging and the monitoring of ROS/RNS levels, which broadens the applications of AgNCs and provides a new tool for related biological investigations.