Male and female gamete abortions, and reduced affinity between the uniting gametes as the causes for sterility in an indica/japonica hybrid in rice
Male and female gamete abortions, and reduced affinity between the uniting gametes as the causes for sterility in an indica/japonica hybrid in rice
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DOI：
10.1007/s00497-004-0214-z
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发表时间：
2004-07-01
影响因子：
--
通讯作者：
Zhang, QF
中科院分区：
文献类型：
--
作者：
Liu, HY;Xu, CG;Zhang, QF
杂种不育性常发生在亚洲栽培稻的籼粳亚种间的杂交中。本研究以籼/粳杂种和籼/籼杂种为材料，研究了雌雄配子形成和发育的细胞学过程及其在受精过程中的相互作用。发现籼粳杂种产生的小孢子中有50%以上不能发育成功能花粉。籼粳杂种小孢子败育率明显高于籼籼杂种。败育胚囊约占籼粳杂种胚囊总数的70%，也远高于籼籼杂种。此外，用粳稻品种的花粉人工授粉后，籼稻品种柱头上的花粉粘附量远低于籼/籼授粉，而自然自花授粉后，籼/籼杂交种柱头上的花粉粘附量远高于籼/粳杂交种。籼粳杂种授粉后也遇到了花粉管生长困难的问题，籼粳杂种的受精率远低于籼籼杂种。这些结果清楚地说明了水稻亚种间杂种不育机制的复杂性，包括合子前和合子后生殖隔离机制。
Hybrid sterility frequently occurs in crosses between indica and japonica subspecies of Asian cultivated rice. In this study, we investigated the cytological processes involved in formation and development of male and female gametes as well as their interactions in fertilization, using an indica/japonica hybrid in comparison with an indica/indica hybrid. It was found that more than 50% of the microspores generated in the indica/japonica hybrid could not develop into functional pollen. The abortion rate of microspores in the indica/japonica hybrid was much higher than that in the indica/indica hybrid. Abortive embryo sacs made up roughly 70% of the embryo sacs examined in the indica/japonica hybrid, which was also much higher than that detected in the indica/indica hybrid. Moreover, the amount of pollen adherence on stigmas of the indica variety upon hand-pollination with pollen from the japonica variety was much lower than the indica/indica pollination, and the number of pollen adhered on the stigmas by natural self-pollination was much greater in the indica/indica hybrid than in the indica/japonica hybrid. The indica/japonica hybrid also encountered difficulties in pollen tube growth after pollination, and the fertilization rate of the indica/japonica hybrid was much lower than that of the indica/indica hybrid. These results clearly illustrate the complexity of the mechanisms underlying inter-subspecific hybrid sterility in rice involving both pre- and post-zygotic reproductive isolation mechanisms.