Static and time-resolved synchrotron small-angle x-ray scattering studies of lyotropic lipid mesophases, model biomembranes and proteins in solution
Static and time-resolved synchrotron small-angle x-ray scattering studies of lyotropic lipid mesophases, model biomembranes and proteins in solution
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DOI：
10.1088/0953-8984/16/5/002
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发表时间：
2004-02-11
影响因子：
2.7
通讯作者：
Köhling, R
中科院分区：
文献类型：
--
作者：
Winter, R;Köhling, R
在这项工作中，我们讨论了使用小角度x射线散射方法来研究软凝聚态物质的温度和压力依赖结构和相行为，特别是生物分子系统，如脂质中间相，模型生物膜系统以及溶液中的蛋白质。除了温度之外，压力也被用作这些研究中的物理参数，特别是用于研究这些系统的能量学和相行为，但也因为高压是某些自然环境的特征，因为生物分子的高压相行为对生物技术应用也很重要。利用压力跳变弛豫技术结合时间分辨同步加速器小角x射线散射，可以研究生物分子相变动力学。我们将该技术应用于研究脂质相变和蛋白质展开/再折叠反应。在讨论了基本的理论概念之后，提出并讨论了几个典型的例子。
In this work we discuss the use of small-angle x-ray scattering methods for investigating the temperature and pressure dependent structure and phase behaviour of soft condensed matter and in particular of biomolecular systems, such as lipid mesophases, model biomembrane systems as well as proteins in solution. In addition to temperature, pressure has also been used as a physical parameter in these studies, in particular for studying the energetics and phase behaviour of these systems, but also because high pressure is a feature of certain natural environments and because the high pressure phase behaviour of biomolecules is also of importance for biotechnological applications. By using the pressure jump relaxation technique in combination with time-resolved synchrotron small-angle x-ray scattering, the kinetics of biomolecular phase transitions can be investigated. We applied the technique for studying lipid phase transitions and protein unfolding/refolding reactions. After the discussion of the underlying theoretical concepts, several characteristic examples are presented and discussed.