Umbilical cord and placental blood hematopoietic stem cells: collection, cryopreservation, and storage.
Umbilical cord and placental blood hematopoietic stem cells: collection, cryopreservation, and storage.
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DOI：
10.1089/scd.1.1992.1.167
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发表时间：
1992
期刊：
影响因子：
--
通讯作者：
J Wagner;H. Broxmeyer;S. Cooper
中科院分区：
文献类型：
--
作者：
J Wagner;H. Broxmeyer;S. Cooper
人脐带和胎盘血液是造血干细胞的丰富来源，可用于强化清髓治疗后重建造血。因此，有必要开发以下技术：(i)无菌收集大量脐带，（ii）优化冷冻保存和解冻后活细胞的产量，以及（iii）最大限度地降低母体t细胞污染的风险。我们对人类脐带和胎盘血的收集、冷冻保存、储存和运输的方案进行了详细描述。
Human umbilical cord and placental blood is a rich source of hematopoietic stem cells that can be used to reconstitute hematopoiesis after intensive myeloablative therapy. Therefore, it has become necessary to develop techniques for (i) collecting large volumes of umbilical cord aseptically, (ii) optimizing the yield of viable cells after cryopreservation and thawing, and (iii) minimizing the risk of maternal T-cell contamination. Our protocol for the collection, cryopreservation, storage, and transport of human umbilical cord and placental blood is described in detail.