Direct electrochemistry of glucose oxidase immobilized on a hexagonal mesoporous silica-MCM-41 matrix
Direct electrochemistry of glucose oxidase immobilized on a hexagonal mesoporous silica-MCM-41 matrix
复制标题
固定在六方介孔二氧化硅-MCM-41基质上的葡萄糖氧化酶的直接电化学
DOI：
10.1016/j.bioelechem.2006.09.009
复制
发表时间：
2007-05-01
影响因子：
5
通讯作者：
Bao, J. C.
中科院分区：
文献类型：
--
作者：
Dai, Z. H.;Ni, J.;Bao, J. C.
研究了葡萄糖氧化酶(GOD)在六方介孔二氧化硅修饰玻碳电极上的直接电化学行为。在0.1M pH 6.1磷酸盐缓冲溶液(PBS)中，吸附的GOD有一对氧化还原峰，峰电位为-417 mV。响应表现为两电子转移与两质子转移反应相耦合的扩散控制电极过程。将GOD固定在六方介孔二氧化硅上，保持了其生物活性和稳定性。此外，在N-2饱和溶液中，以二茂铁一元羧酸(FMCA)为介体，固定化GOD能够电催化葡萄糖氧化生成葡萄糖内酯，表明该电极在葡萄糖生物传感器中具有潜在的应用前景。该传感器可以排除常见共存的尿酸、对乙酰氨基酚和抗坏血酸的干扰，快速、灵敏地诊断糖尿病。这项工作表明，介孔二氧化硅为蛋白质固定化和生物传感器的构建提供了一种新的基质。(C)2006年爱思唯尔BN。版权所有。
The direct electrochemistry of glucose oxidase (GOD) immobilized on a hexagonal mesoporous silica modified glassy carbon electrode was investigated. The adsorbed GOD displayed a pair of redox peaks with a formal potential of -417 mV in 0.1 M pH 6.1 phosphate buffer solution (PBS). The response showed a diffusion-controlled electrode process with a two-electron transfer coupled with a two-proton transfer reaction process. GOD immobilized on a hexagonal mesoporous silica retained its bioactivity and stability. In addition, the immobilized GOD could electrocatalyze the oxidation of glucose to gluconlactone by taking ferrocene monocarboxylic acid (FMCA) as a mediator in N-2 saturated solutions, indicating that the electrode may have the potential application in biosensors to analyze glucose. The sensor could exclude the interference of commonly coexisted uric acid, p-acetaminophenol and ascorbic acid and diagnose diabetes very fast and sensitively. This work demonstrated that the mesoporous silica provided a novel matrix for protein immobilization and the construction of biosensors. (C) 2006 Elsevier BN. All rights reserved.