Plasmatocyte spreading peptide is encoded by an mRNA differentially expressed in tissues of the moth Pseudoplusia includens
Plasmatocyte spreading peptide is encoded by an mRNA differentially expressed in tissues of the moth Pseudoplusia includens
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DOI：
10.1006/bbrc.1998.9145
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发表时间：
1998-09-18
影响因子：
3.1
通讯作者：
Strand, MR
中科院分区：
文献类型：
--
作者：
Clark, KD;Witherell, A;Strand, MR
血细胞(血细胞)是无脊椎动物免疫系统的重要组成部分，这一点早已为人所知，但对调节其功能的分子却知之甚少。最近，我们从褐飞蛾中发现了一种浆细胞扩散肽(PSP1)，它调节一种称为浆细胞的血细胞亚类的运输和黏附。在这里，我们报告了一个编码PSP1前体蛋白的cDNA(P15)的克隆。Northern印迹分析表明，PSP1前体在脂肪体中表达，其他PSP1相关转录本在神经组织和血细胞中表达。体外转录/翻译耦合反应表明，p15产生一种可被PSP1多克隆抗体识别的蛋白质。免疫印迹实验进一步表明，PSP1原蛋白可能存在于包括血浆和脂肪体在内的磷虾体内。(C)1998年学术出版社。
It has long been known that blood cells (hemocytes) are an essential component of the invertebrate immune system, yet little is known about the molecules mediating their function. Recently, we identified plasmatocyte spreading peptide (PSP1) from the moth Pseudoplusia includens which regulates the trafficking and adhesion of a hemocyte subclass called plasmatocytes. Here, we report the cloning of a cDNA (p15) that encodes a PSP1 precursor protein. Northern blot analysis revealed that a homologous prepro-PSP1 mRNA is expressed in fat body, and that other PSP1-related transcripts are expressed in nervous tissue and hemocytes. Coupled in vitro transcription/translation reactions indicated that p15 produces a protein recognized by a PSP1 polyclonal antibody. Immunoblotting experiments further revealed that a putative pro-PSP1 protein is present in P. includens plasma and fat body. (C) 1998 Academic Press.