A microalgal-based preparation with synergistic cellulolytic and detoxifying action towards chemical-treated lignocellulose.
A microalgal-based preparation with synergistic cellulolytic and detoxifying action towards chemical-treated lignocellulose.
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DOI：
10.1111/pbi.13447
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发表时间：
2021-01
影响因子：
13.8
通讯作者：
Dall'Osto L
中科院分区：
文献类型：
--
作者：
Benedetti M;Barera S;Longoni P;Guardini Z;Herrero Garcia N;Bolzonella D;Lopez-Arredondo D;Herrera-Estrella L;Goldschmidt-Clermont M;Bassi R;Dall'Osto L
木质纤维素在高温下转化为可发酵糖的过程已经引起了可再生化学品和生物燃料高效生产的关注，因为在高温下竞争的微生物活动被抑制，而且耐热性细胞壁降解酶优于中温性酶。在这里，我们报道了在莱茵衣藻叶绿体中产生四种不同的耐热细胞壁降解酶的平台的开发。该混合酶由C. saccharolyticus的纤维素生物水解酶CBM3GH5、P. furiosus的β -葡萄糖苷酶celB、T. neapolitana的内切葡聚糖酶B和内切木聚糖酶XynA组成。此外，我们还设计了转质体微藻，用于表达来自假单胞菌的亚磷酸酯脱氢酶D，使其能够通过选择性亚磷酸酯营养在非无菌培养基中生长。由糖苷水解酶（GH）结构域GH12/GH5/GH1组成的纤维素水解混合物可以将碱性处理的木质纤维素转化为葡萄糖，反应48小时后效率为14%至17%，酶负荷为0.05% （w/w）。转基因微藻提取物处理后的纤维素水解产物促进了产甲烷菌的沼气产量和产油微藻小球藻的混合营养生长。值得注意的是，微藻处理抑制了生物质碱性处理释放的抑制副产物的有害影响，从而允许C. vulgaris在混合营养生长下有效地同化木质纤维素衍生的糖。
High‐temperature bioconversion of lignocellulose into fermentable sugars has drawn attention for efficient production of renewable chemicals and biofuels, because competing microbial activities are inhibited at elevated temperatures and thermostable cell wall degrading enzymes are superior to mesophilic enzymes. Here, we report on the development of a platform to produce four different thermostable cell wall degrading enzymes in the chloroplast of Chlamydomonas reinhardtii. The enzyme blend was composed of the cellobiohydrolase CBM3GH5 from C. saccharolyticus, the β‐glucosidase celB from P. furiosus, the endoglucanase B and the endoxylanase XynA from T. neapolitana. In addition, transplastomic microalgae were engineered for the expression of phosphite dehydrogenase D from Pseudomonas stutzeri, allowing for growth in non‐axenic media by selective phosphite nutrition. The cellulolytic blend composed of the glycoside hydrolase (GH) domain GH12/GH5/GH1 allowed the conversion of alkaline‐treated lignocellulose into glucose with efficiencies ranging from 14% to 17% upon 48h of reaction and an enzyme loading of 0.05% (w/w). Hydrolysates from treated cellulosic materials with extracts of transgenic microalgae boosted both the biogas production by methanogenic bacteria and the mixotrophic growth of the oleaginous microalga Chlorella vulgaris. Notably, microalgal treatment suppressed the detrimental effect of inhibitory by‐products released from the alkaline treatment of biomass, thus allowing for efficient assimilation of lignocellulose‐derived sugars by C. vulgaris under mixotrophic growth.
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影响因子：
6.3
作者：
Castiglia D;Sannino L;Marcolongo L;Ionata E;Tamburino R;De Stradis A;Cobucci-Ponzano B;Moracci M;La Cara F;Scotti N
通讯作者：
Scotti N
来自Trichoderma Reesei的Bobiobiohydrollose 1在单核生物步骤中降解了纤维素。
影响因子：
16.6
作者：
Brady SK;Sreelatha S;Feng Y;Chundawat SP;Lang MJ
通讯作者：
Lang MJ
影响因子：
8.7
作者：
Battista, Federico;Fino, Debora;Ruggeri, Bernardo
通讯作者：
Ruggeri, Bernardo
影响因子：
4.1
作者：
Benedetti, Manuel;Vecchi, Valeria;Dall'Osto, Luca
通讯作者：
Dall'Osto, Luca
影响因子：
6.3
作者：
Dall'Osto, Luca;Cazzaniga, Stefano;Bassi, Roberto
通讯作者：
Bassi, Roberto