Gender-Dimorphic Regulation of Skeletal Muscle Proteins in Streptozotocin-Induced Diabetic Rats
Gender-Dimorphic Regulation of Skeletal Muscle Proteins in Streptozotocin-Induced Diabetic Rats
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DOI：
10.1159/000343378
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发表时间：
2013-01-01
影响因子：
--
通讯作者：
Yun, Jong Won
中科院分区：
文献类型：
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作者：
Choi, Minji;Choi, Jung-Won;Yun, Jong Won
工作背景：尽管事实上，性别差异增加糖尿病的风险，并有助于性别特异性护理的需要，仍然存在矛盾的结果，是否性别二型增加1型糖尿病的发展的易感性。研究方法：为了研究健康对照组和STZ诱导的糖尿病大鼠骨骼肌蛋白质的性别二态性调节，我们采用双向电泳结合质谱法进行了差异蛋白质组分析。结果如下：动物实验表明，STZ治疗使雌性大鼠比其雄性同窝出生的大鼠更容易诱发糖尿病，由于激素调节，血浆胰岛素水平显著降低。骨骼肌蛋白质组学分析确定了共21个蛋白质显示性别二态性差异表达模式之间的健康对照组和糖尿病大鼠。最有趣的是，性别特异性蛋白质组比较显示，雄性和雌性大鼠显示出参与肌肉收缩，碳水化合物和脂质代谢，以及氧化磷酸化和细胞应激的蛋白质的差异调节。结论：目前的蛋白质组学研究表明，受损的蛋白质调节在雌性糖尿病大鼠的肌肉组织中更为突出，这更容易受到STZ诱导的糖尿病。我们期望目前的蛋白质组学数据可以为糖尿病的循证性别特异性治疗提供有价值的信息。版权所有（C）2013 S. Karger AG，巴塞尔
Background: Despite the fact that sexual differences increase diabetic risk and contribute to the need for gender-specific care, there remain contradictory results as to whether or not sexual dimorphism increases susceptibility to the development of type 1 diabetes mellitus. Methods: To examine gender-dimorphic regulation of skeletal muscle proteins between healthy control and STZ-induced diabetic rats of both genders, we performed differential proteome analysis using two-dimensional electrophoresis combined with mass spectrometry. Results: Animal experiments revealed that STZ treatment rendered female rats more susceptible to induction of diabetes than their male littermates with significantly lower plasma insulin levels due to hormonal regulation. Proteomic analysis of skeletal muscle identified a total of 21 proteins showing gender-dimorphic differential expression patterns between healthy controls and diabetic rats. Most interestingly, gender-specific proteome comparison showed that male and female rats displayed differential regulation of proteins involved in muscle contraction, carbohydrate, and lipid metabolism, as well as oxidative phosphorylation and cellular stress. Conclusion: The current proteomic study revealed that impaired protein regulation was more prominent in the muscle tissue of female diabetic rats, which were more susceptible to STZ-induced diabetes. We expect that the present proteomic data can provide valuable information for evidence-based gender-specific treatment of diabetes. Copyright (C) 2013 S. Karger AG, Basel