DEVELOPMENTAL CONSEQUENCES OF AWDB3, A CELL-AUTONOMOUS LETHAL MUTATION OF DROSOPHILA INDUCED BY HYBRID DYSGENESIS
DEVELOPMENTAL CONSEQUENCES OF AWDB3, A CELL-AUTONOMOUS LETHAL MUTATION OF DROSOPHILA INDUCED BY HYBRID DYSGENESIS
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DOI：
10.1016/0012-1606(88)90170-4
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发表时间：
1988-09-01
影响因子：
2.7
通讯作者：
SHEARN, A
中科院分区：
文献类型：
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作者：
DEAROLF, CR;HERSPERGER, E;SHEARN, A
为了恢复影响象盘的突变，使相关基因易于克隆，对导致幼虫晚期/蛹早期致死的突变进行了杂交异常筛选。本文介绍了诱变过程和恢复突变引起的表型。在81例晚期幼虫/蛹致死性突变中，有20例可引起影像学椎间盘缺损。这20个突变定义了12个不同的基因。本文还介绍了由这12个被命名为异常翅盘（awd）的基因中的一个突变引起的发育缺陷；下面的论文（C. Dearolf， N. Tripoulas， J. Biggs, and A. sheen, 1988, Dev. Biol. 129, 169-178）描述了awd基因的克隆及其转录模式的分析。Awdb3纯合子以正常的速度发育，直到第二幼虫期结束，这时它们的发育速度降低。经过第三个幼虫期后，它们形成蛹并死亡。突变翼盘形态异常，细胞大量死亡。这些异常的翅盘，以及看起来形态正常的腿和眼天线盘，在注射到变态幼虫体内时分化很差或根本不分化。基因嵌合体分析表明，awdb3突变以细胞自主方式在翅膀、腿和眼天线盘中表达。awdb3纯合子三龄幼虫的脑和前脑室由于脂滴的积累而呈空泡状。虽然突变的生殖细胞能够产生正常的卵，但当注入野生型幼虫时，突变的卵巢无法发育。
In order to recover mutations affecting imaginal discs in a way would allow the relevant genes to be readily cloned, a hybrid dysgenic screen was performed for mutations causing late larval/early pupal lethality. This paper describes that mutagenesis procedure and the phenotypes caused by the mutations that were recovered. Of 81 late larval/pupal lethal mutations that were recovered, 20 cause imaginal disc defects. These 20 mutations define 12 different genes. This paper also includes a description of the developmental defects caused by a mutation in one of those 12 genes which have named abnormal wing discs (awd); the following paper (C. Dearolf, N. Tripoulas, J. Biggs, and A. Shearn, 1988, Dev. Biol. 129, 169-178) describes the cloning of the awd gene and an analysis of its pattern of transcription. awdb3 homozygotes develop at a normal rate until the end of the second larval instar, when their rate of development is reduced. After an extended third larval instar, they form puparia and die. Mutant wing discs have an abnormal morphology and extensive cell death. These abnormal wing discs, and also the leg and eye-antenna discs which appear to be morphologically normal, differentiate poorly or not at all when injected into metamorphosing larvae. Analysis of genetic mosaics indicates that the awdb3 mutation is expressed in a cell-autonomous manner in wing, leg, and eye-antenna discs. The larval brain and proventriculus in awdb3 homozygous third-instar larvae appear to be vacuolated due to the accumulation of lipid droplets. Mutant ovaries are unable to develop when injected into wild-type larvae, although mutant germ cells are capable of producing normal eggs.