Ecklonia cava polyphenol protects the liver against ethanol-induced injury in rats
Ecklonia cava polyphenol protects the liver against ethanol-induced injury in rats
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DOI：
10.1016/j.bbagen.2012.02.008
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发表时间：
2012-07-01
影响因子：
3
通讯作者：
Kojima-Yuasa, Akiko
中科院分区：
文献类型：
--
作者：
Takahashi, Mai;Satake, Naoko;Kojima-Yuasa, Akiko
背景：酒精性肝病的发生发展是一个复杂的过程，涉及肝脏的实质细胞和非实质细胞。方法：将分离的肝细胞和肝星状细胞(HSCs)与乙醇共同孵育。在用乙醇孵育的培养物中加入多酚(ECP)。雄性Wistar大鼠饲喂含0.02%或0.2%ECP的饲料或不含ECP的饲料。结果：在体外培养的肝细胞中，ECP通过维持细胞内谷胱甘肽(GSH)水平，抑制乙醇诱导的肝细胞死亡。在HSCs中，ECP处理通过维持细胞内活性氧和GSH水平来抑制乙醇诱导的I型胶原和α-平滑肌肌动蛋白表达的增加。我们研究了ECP对乙醇和CCl4处理的大鼠血清AST和ALT活性的影响，以及肝纤维化的进展。ECP处理可抑制乙醇和CCl4处理大鼠的血浆AST和ALT活性。ECP对乙醇和CCl4诱导的大鼠肝损伤有完全的保护作用。一般意义：ECP可能是预防酒精性肝损伤的候选药物。(C)2012爱思唯尔B.V.保留所有权利。
Background: The development of alcoholic liver disease is a complex process that involves both the parenchymal and non-parenchymal cells of the liver. We examined the effect of an Ecklonia cava extract on ethanol-induced liver injury.Methods: Isolated hepatocytes and hepatic stellate cells (HSCs) were incubated with ethanol. Ecklonia cava polyphenol (ECP) was added to the cultures that had been incubated with ethanol. Male Wistar rats were fed a diet that included 0.02% or 0.2% ECP or no ECP. For a period of 3 weeks, the animals were given drinking water containing 5% ethanol and were also treated with carbon tetrachloride (CCl4) (0.1 ml/kg of body weight).Results: In the cultured hepatocytes, the ECP treatment suppressed the ethanol-induced increase in cell death by maintaining intracellular glutathione (GSH) levels. In HSCs, ECP treatment suppressed the ethanol-induced increases in type I collagen and a-smooth muscle actin expression by maintaining intracellular levels of reactive oxygen species and GSH. We examined the effects of ECP on serum AST and ALT activity, as well as the progression of liver fibrosis in rats treated with ethanol and CCl4. ECP treatment suppressed plasma AST and ALT activities in the ethanol- and CCl4-treated rats. ECP treatment fully protected the rats against ethanol- and CCl4-induced liver injury.General significance: ECP may be a candidate for preventing ethanol-induced liver injury. (C) 2012 Elsevier B.V. All rights reserved.