Gene Expression Signature and the Prediction of Lymph Node Metastasis in Colorectal Cancer by DNA Microarray
Gene Expression Signature and the Prediction of Lymph Node Metastasis in Colorectal Cancer by DNA Microarray
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DOI：
10.1007/dcr.0b013e3181b53684
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发表时间：
2009-12
影响因子：
3.9
通讯作者：
Toshiaki Watanabe;T. Kobunai;Toshiaki Tanaka;S. Ishihara;K. Matsuda;H. Nagawa
中科院分区：
文献类型：
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作者：
Toshiaki Watanabe;T. Kobunai;Toshiaki Tanaka;S. Ishihara;K. Matsuda;H. Nagawa
淋巴结转移是决定结直肠癌预后的重要因素。如果我们能在手术前预测淋巴结转移的存在，它可能有助于决定是否需要手术淋巴结清扫或其他术前治疗方式，可能会提高生存率。我们的目的是确定一组可用于表征和预测淋巴结转移的鉴别基因。方法：89例结直肠癌患者进行了研究。采用人类U133 Plus 2.0 GeneChip®检测肿瘤基因表达谱，并对有无淋巴结转移的患者进行比较。结果：我们鉴定了73个新的鉴别基因，其表达在有无淋巴结转移的患者之间存在显著差异。利用这个基因集，我们能够建立一个新的模型来预测淋巴结转移的存在，准确率为88.4%。识别基因与多种功能相关，包括受体活性和转录调节活性。这些基因包括跨膜糖蛋白，据报道它与前列腺癌的淋巴结转移密切相关。Transmembrane糖蛋白表现出显着较高的表达在患者淋巴结transferation.CONCLUSIONS：本研究表明，基因表达谱可能是有用的，在预测淋巴结转移的存在。因此，基因表达谱可以帮助建立针对结直肠癌的个性化定制治疗，并为开发新的治疗靶点提供见解。
PURPOSE:Lymph node metastasis is an important factor in determining the outcome of colorectal cancer. If we can predict the presence of lymph node metastasis before surgery, it may help in deciding the need for surgical lymph node dissection or additional preoperative treatment modalities that might improve survival. Our objective here was to identify a set of discriminating genes that can be used for characterization and prediction of lymph node metastasis.METHODS:Eighty-nine colorectal cancer patients were studied. Gene expression profiles of cancer were determined by human U133 Plus 2.0 GeneChip®, and patients with and without lymph node metastasis were compared.RESULTS:We identified 73 novel discriminating genes in which expression was significantly different between patients with and without lymph node metastasis. Using this gene set, we were able to establish a new model to predict the presence of lymph node metastasis with an accuracy of 88.4%. Discriminating genes were associated with various functions, including receptor activity and transcription regulatory activity. The list of genes included transmembrane glycoprotein, which has been reported to have a close relationship with lymph node metastasis in prostate cancer. Transmembrane glycoprotein showed significantly higher expression in patients with lymph node metastasis.CONCLUSIONS:The present study suggests the possibility that gene expression profiling may be useful in predicting the presence of lymph node metastasis. Thus, gene expression profiling could help to establish individualized tailored therapy for colorectal cancer and provide insights into the development of novel therapeutic targets.