Distribution of keratin 8-containing cell clusters in mouse embryonic tongue: Evidence for a prepattern for taste bud development
Distribution of keratin 8-containing cell clusters in mouse embryonic tongue: Evidence for a prepattern for taste bud development
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DOI：
10.1002/cne.10551
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发表时间：
2003-03-03
影响因子：
2.5
通讯作者：
Roberts, JD
中科院分区：
文献类型：
--
作者：
Mbiene, JP;Roberts, JD
味觉乳头形态发生的起始不依赖于神经支配。为了解决味蕾形成是否与味觉乳头形态发生相关的问题，我们研究了从胚胎第11天到出生的小鼠胚胎中发育的舌头。尽管妊娠13天时舌背表面形态光滑，但我们观察到胚胎味蕾原基是由细胞角蛋白8阳性的细胞组成的离散集合，且在舌前部的上皮细胞中呈细长状。在随后的发育阶段，细胞角蛋白8继续在胚胎味蕾中表达，这些味蕾以规则的间隔沿着对称地位于舌背前发育的正中沟两侧的点状图案分布。从妊娠15.5d至出生，发育中的环状乳突内可见胚胎性味蕾。当胚胎味蕾第一次出现时，舌背上皮并没有受到神经支配。在发育中的菌状乳头中，胚胎味蕾的数量增加，直到出生，与上皮细胞的神经侵袭无关。因此，味蕾更有可能发生在产前，而不依赖于神经支配。(C)2003年Wiley-Liss，Inc.
The initiation of the morphogenesis of gustatory papillae is independent of innervation. To address the question of whether taste bud formation is associated with gustatory papilla morphogenesis, we examined developing tongues in mouse embryos from embryonic day 11 to birth. Despite the smooth morphological appearance of the lingual dorsal surface at 13 days of gestation, we observed embryonic taste bud primordia as discrete collections of cytokeratin 8-positive and elongated cells in epithelial placodes in the anterior tongue. In subsequent stages until birth, cytokeratin 8 continues to be expressed in embryonic taste buds distributed in punctuate patterns at regular intervals along rows that are symmetrically located on both sides of the median sulcus in the dorsal anterior developing tongue. Embryonic taste buds were observed in the developing circumvallate papillae from 15.5 days of gestation until birth. The dorsal epithelium of the anterior tongue is not innervated when embryonic taste buds first occur. The increased numbers of embryonic taste buds in developing fungiform papillae until birth are not correlated with the neural invasion of the epithelium. Thus, taste buds occur prenatally more likely independently of the innervation. (C) 2003 Wiley-Liss, Inc.