Proteomic Analysis of the Peri-Infarct Area after Human Umbilical Cord Mesenchymal Stem Cell Transplantation in Experimental Stroke.
Proteomic Analysis of the Peri-Infarct Area after Human Umbilical Cord Mesenchymal Stem Cell Transplantation in Experimental Stroke.
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实验性脑卒中人脐带间充质干细胞移植后梗塞周围区域的蛋白质组学分析
DOI：
10.14336/ad.2016.0121
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发表时间：
2016-10
影响因子：
7.4
通讯作者：
Yun X
中科院分区：
文献类型：
--
作者：
Dongsheng H;Zhuo Z;Jiamin L;Hailan M;Lijuan H;Fan C;Dan Y;He Z;Yun X
在众多治疗中风的方法中，人脐带间充质干细胞(Huc-MSCs)治疗已取得了一些可喜的结果。然而，潜在的机制仍不清楚。我们分析了经Huc-MSCs治疗后的缺血性卒中后大脑皮质梗死周区域的蛋白质表达谱，发现有16个蛋白质在接受或不接受Huc-MSCs治疗的组中表达差异。通过基因本体论术语分析和与CD200-CD200R1、CCL21-CXCR3和转录因子的网络进一步确定了这些蛋白。其中Abca13、Grb2和PTGDS用定量聚合酶链式反应和酶联免疫吸附试验进行了验证。我们发现Abca13的蛋白水平和Grb2的mRNA水平与蛋白质组学分析的结果一致。最后，对这些蛋白质的功能进行了描述，并指出了值得进一步研究的潜在蛋白质。我们的数据可能为使用Huc-MSCs治疗中风提供可能的潜在机制。
Among various therapeutic approaches for stroke, treatment with human umbilical cord mesenchymal stem cells (hUC-MSCs) has acquired some promising results. However, the underlying mechanisms remain unclear. We analyzed the protein expression spectrum of the cortical peri-infarction region after ischemic stroke followed by treatment with hUC-MSCs, and found 16 proteins expressed differentially between groups treated with or without hUC-MSCs. These proteins were further determined by Gene Ontology term analysis and network with CD200-CD200R1, CCL21-CXCR3 and transcription factors. Three of them: Abca13, Grb2 and Ptgds were verified by qPCR and ELISA. We found the protein level of Abca13 and the mRNA level of Grb2 consistent with results from the proteomic analysis. Finally, the function of these proteins was described and the potential proteins that deserve to be further studied was also highlighted. Our data may provide possible underlying mechanisms for the treatment of stroke using hUC-MSCs.