Monocyte chemotactic protein-1 in proliferative vitreoretinal disorders
Monocyte chemotactic protein-1 in proliferative vitreoretinal disorders
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DOI：
10.1016/s0002-9394(14)71072-4
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发表时间：
1997-05-01
影响因子：
4.2
通讯作者：
Missotten, L
中科院分区：
文献类型：
--
作者：
AbuElAsrar, AM;VanDamme, J;Missotten, L
目的：研究趋化因子单核细胞趋化蛋白 1 (MCP-1) 和白细胞介素 8 (IL-8) 是否参与增殖性玻璃体视网膜疾病的发病机制，并研究它们与 IL-6 之间可能的相互作用。 方法：在一项对 125 名连续患者（125 只眼睛）进行的前瞻性研究中，获取了玻璃体和配对血清样本，并进行了 MCP-1 和 MCP-1 的检测。 IL-8。 IL-6 水平通过 IL-6 依赖性杂交瘤细胞系 7TD1 的增殖来测定。 结果：在视网膜脱离患者的 27 份玻璃体样本中的 13 份（48%）、黄斑皱褶患者的 8 份样本中的 5 份（63%）、增殖性视网膜脱离患者的 43 份样本中的 31 份（72%）中检测到单核细胞趋化蛋白-1。 玻璃体视网膜病变的 42 份样本中有 32 份 (76%)，但在 5 名特发性视网膜前膜患者的样本中没有，视网膜脱离、黄斑皱褶和增殖性玻璃体视网膜病变组中 MCP-1 检测的发生率与玻璃体视网膜病变的严重程度之间存在显着 (P = .049) 相关性。 扩散。在来自患有视网膜脱离的眼睛的两份玻璃体样本、来自患有增殖性玻璃体视网膜病变的眼睛的两份样本以及来自患有增殖性糖尿病视网膜病变的眼睛的三份样本中检测到白细胞介素-8。玻璃体样本中的单核细胞趋化蛋白-1 水平与 IL-6 水平呈正相关（r = .31，P = .01）。与未检测到 MCP-1 水平的玻璃体样本相比，白细胞介素 6 水平显着升高 (P = 0.0097)。 结论：患有增殖性玻璃体视网膜疾病的眼睛的玻璃体样本中相当大比例存在单核细胞趋化蛋白-1，并且可能有助于刺激单核细胞和巨噬细胞浸润到患有这些疾病的眼睛中。
PURPOSE: To investigate whether the chemokines monocyte chemotactic protein-1 (MCP-1) and interleukin-8 (IL-8) are involved in the pathogenesis of proliferative vitreoretinal disorders and to study their possible interaction with IL-6.METHODS: In a prospective study of 125 consecutive patients (125 eyes), vitreous and paired serum samples were obtained and were assayed for MCP-1 and IL-8. Levels of IL-6 were determined by proliferation of the IL-6-dependent hybridoma cell line 7TD1.RESULTS: Monocyte chemotactic protein-1 was detected in 13 (48%) of 27 vitreous samples from patients with retinal detachment, in five (63%) of eight samples from patients with macular pucker, in 31 (72%) of 43 samples from patients with proliferative vitreoretinopathy, and in 32 (76%) of 42 samples from patients with proliferative diabetic retinopathy, but not in samples from five patients with idiopathic epiretinal membrane, There was a significant (P = .049) correlation between the incidence of MCP-1 detection in retinal detachment, macular pucker, and proliferative vitreoretinopathy groups and the severity of proliferation. Interleukin-8 was detected in two vitreous samples from eyes with retinal detachment, in two samples from eyes with proliferative vitreoretinopathy, and in three samples from eyes with proliferative diabetic retinopathy. Monocyte chemotactic protein-1 levels in the vitreous samples were positively correlated with IL-6 levels (r = .31, P = .01). Interleukin-6 levels were significantly (P = .0097) greater in vitreous samples with than without detectable levels of MCP-1.CONCLUSION: Monocyte chemotactic protein-1 is present in a substantial percent of vitreous samples from eyes with proliferative vitreoretinal disorders and may help in stimulating the infiltration of monocytes and macrophages into eyes with these disorders.