Simultaneous determination of three curcuminoids in Curcuma longa L. by high performance liquid chromatography coupled with electrochemical detection.
Simultaneous determination of three curcuminoids in Curcuma longa L. by high performance liquid chromatography coupled with electrochemical detection.
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DOI：
10.1016/j.jpha.2013.10.002
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发表时间：
2014-10
影响因子：
8.8
通讯作者：
Chen Z
中科院分区：
文献类型：
--
作者：
Long Y;Zhang W;Wang F;Chen Z
建立了HPLC -电化学联用分析姜黄中姜黄素、去甲氧基姜黄素和双去甲氧基姜黄素三种有效成分的新方法。三种姜黄素在C18色谱柱上分离良好，检测灵敏度高。流动相为乙腈和10 mM Na2HPO4-H3PO4 (pH 5.0) （50:50, v/v）。姜黄素、去甲氧基姜黄素和双去甲氧基姜黄素分别在0.208 ~ 41.6 μM、0.197 ~ 39.4 μM和0.227 ~ 114 μM范围内呈良好的线性关系。其检出限为10 ~ 8 M，低于紫外法。日内精密度相对标准偏差（rsd）为1.06% ~ 1.88%，日内精密度为4.30% ~ 5.79%。该方法应用于药材样品中，回收率为86.3% ~ 111%。
A novel method for analysis of three active components curcumin, demethoxycurcumin and bisdemethoxycurcumin in Curcuma longa L. was developed by HPLC coupled with electrochemical detection. Three curcuminoids were well separated on a C18 column and detected with high sensitivity. A mobile phase containing acetonitrile and 10 mM Na2HPO4–H3PO4 (pH 5.0) (50:50, v/v) was used. Good linearity was obtained in the range of 0.208–41.6, 0.197–39.4, and 0.227–114 μM for curcumin, demethoxycurcumin and bisdemethoxycurcumin respectively. The limit of detection reached up to 10−8 M, which was lower than that by UV detection. The relative standard deviations (RSDs) ranged from 1.06% to 1.88% for intra-day precision and from 4.30% to 5.79% for inter-day precision, respectively. The proposed method has been applied in real herb sample and recoveries ranging from 86.3% to 111% were obtained.