Genetic basis of long shelf life and variability into Penjar tomato
Genetic basis of long shelf life and variability into Penjar tomato
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DOI：
10.1007/s10722-011-9677-6
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发表时间：
2012-02-01
影响因子：
2
通讯作者：
Nuez, F.
中科院分区：
文献类型：
--
作者：
Casals, J.;Pascual, L.;Nuez, F.
Penjar番茄是在西班牙东北部栽培的一种品种类型，其特征在于具有长货架期、小果实和品种间的广泛形态变异。为了确定长货架寿命的Penjar品种类型的遗传基础，并描述该组的遗传背景和农业形态学特征，我们研究了27 Penjar加入代表的整个范围内的变化性状相关的农艺行为，果实形态和感官质量。我们发现Penjar的长货架期（平均：126.8天）是由于成熟突变体alcoba double dagger a（alc），这种突变的分子基础是NAC.NOR基因编码序列的317位胸腺嘧啶被腺嘌呤取代;因此，alcoba double dagger a（alc）是非成熟（nor）的等位基因。扩增片段长度多态性（AFLP）的研究发现，18.07%的多态位点内的Penjar品种类型，这是高于通常的地方品种。品种之间的农艺和形态性状变异也很大;因此，Penjar群体的异质性可能是由于在不同地方品种中引入了等位基因alc造成的。我们的研究结果还表明，在种质中含有alc突变货架期与果实大小（重量，宽度，长度和数量的室）呈负相关，因此，小果的品种类型内的优势可能是这个特性被拖沿着在选择长货架期的结果。
Penjar tomato is a varietal type cultivated in northeast Spain that characteristically has a long shelf life, small fruit, and wide morphological variability among cultivars. To determine the genetic basis for the long shelf life in the Penjar varietal type and to describe the genetic background and agromorphologic characteristics of the group, we studied 27 Penjar accessions representative of the entire range of variation in traits related to agronomic behavior, fruit morphology, and sensory quality. We found that the long shelf life of Penjar (mean: 126.8 days) is due to the ripening mutant alcoba double dagger a (alc), and the molecular basis of this mutation is the replacement of thymine by adenine in position 317 of the coding sequence of the NAC.NOR gene; thus, alcoba double dagger a (alc) is an allele of non-ripening (nor). The amplified fragment length polymorphism (AFLP) study found 18.07% polymorphic loci within the Penjar varietal type, which is higher than usual in landraces. The variation in agronomic and morphologic traits between accessions was also very high; thus, the heterogeneity of the Penjar group probably results from the introduction of the allele alc in distinct landraces. Our results also show that in germplasm containing the alc mutation shelf life is negatively correlated with fruit size (weight, width, length, and number of locules); thus, the predominance of small fruit within the varietal type is probably the result of this trait being dragged along in the selection for long shelf life.