Tenascin expression in normal, hyperplastic, dysplastic and neoplastic canine mammary tissues
Tenascin expression in normal, hyperplastic, dysplastic and neoplastic canine mammary tissues
复制标题
DOI：
10.1053/jcpa.2001.0519
复制
发表时间：
2002-01-01
影响因子：
0.8
通讯作者：
Nederbragt, H
中科院分区：
文献类型：
--
作者：
Faustino, AMR;van Garderen, E;Nederbragt, H
乳腺肿瘤是雌性犬最常见的肿瘤，可能具有复杂的组织学模式，上皮细胞和梭形细胞参与转化过程。这些肿瘤的常见特征是细胞外基质的软骨样或骨化生，主要发生在增殖的梭形细胞区域，可能起源于肌上皮。本研究评估了186例犬乳腺组织手术样本中腱生蛋白的表达，范围从正常到肿瘤。腱生蛋白存在于所有研究的乳腺组织中，在重塑情况和肿瘤病变中表达增加。基底膜是最常见的标记结构，但间质组织在肿瘤性病变中更常见和广泛标记。细胞外基质在实体癌和间变性癌以及梭形细胞增生区呈阳性。腱生蛋白在细胞外基质中的表达在初始软骨样化生的区域中也是丰富的，并且在几乎所有的混合性肿瘤的软骨岛中具有可变的延伸。在分化良好的分泌区，只有腔细胞的顶端颗粒呈阳性，这表明在分泌分化过程中的不同模式的tenascin表达。当这两种分子共表达时，硫酸软骨素的消化显着改善了腱生蛋白的标记。虽然我们的研究结果表明，腱生蛋白不能被用作标记的转化或恶性犬乳腺肿瘤，很明显，这种分子在犬乳腺的增殖和分化过程中发挥着重要作用。(C)2002年哈考特出版有限公司
Mammary tumours are the most common neoplasias of female dogs and may have a complex histological pattern with both epithelial and spindle cells participating in the transformation process. A frequent feature of these tumours is chondroid or bone metaplasia of the extracellular matrix, which mainly occurs in areas of proliferated spindle-shaped cells, probably of myoepithelial origin. The present study evaluates immunohistochemically the expression of tenascin in 186 surgical samples of canine mammary tissues, ranging from normality to neoplasia. Tenascin was present in all mammary tissues studied, with an increased expression in remodelling situations and in neoplastic lesions. Basement membrane was the most frequently labelled structure, but stromal tissue was more often and widely labelled in neoplastic lesions. The extracellular matrix was positive in solid and anaplastic carcinomas as well as in spindle cell proliferation areas. Tenascin expression in extracellular matrix was also abundant in areas of initial chondroid metaplasia and, with variable extension, in almost all cartilage islands of mixed tumours. In well differentiated secretory areas only apical granules of luminal cells were positive, suggesting a different pattern of tenascin expression during secretory differentiation. The digestion of chondroitin sulphate significantly improved the labelling for tenascin when a co-expression of these two molecules was present. Although our results suggest that tenascin cannot be used as a marker of transformation or of malignancy in canine mammary oncology, it is clear that this molecule plays an important role in proliferation and differentiation processes in the canine mammary gland. (C) 2002 Harcourt Publishers Ltd.