Molecular characterization of pheromone biosynthesis activating neuropeptide from the diamondback moth, Plutella xylostella (L.)
Molecular characterization of pheromone biosynthesis activating neuropeptide from the diamondback moth, Plutella xylostella (L.)
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DOI：
10.1016/j.peptides.2005.04.016
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发表时间：
2005-12-01
期刊：
影响因子：
3
通讯作者：
Boo, KS
中科院分区：
文献类型：
--
作者：
Lee, DW;Boo, KS
信息素生物合成激活神经肽（PBAN）产生于食管下神经节，刺激信息素腺体产生信息素。从小菜蛾（Plutella xylostella（L.））编码193个氨基酸，包括PBAN，命名为P1 x-PBAN，以及四种其他神经肽（NP）：滞育激素（DH）同源物，α-NP，β-NP和γ-NP。所有的肽在它们的C-末端被酰胺化并且共享保守基序，FXPR（或K）L结构，如从其他PBAN cDNA报道的。P1 x-PBAN由30个氨基酸组成，是迄今为止报道的最短的PBAN。与其他已知的PBAN相比，P1 x-PBAN表现出低于50%的同源性。P1 x-DH同源物在结构上不同于家蚕的DH。P1 x-β-NP（16个氨基酸）的长度是最短的，并且显示出相对低的相似性，而γ-NP（10个氨基酸长度）是所检查的γ-NP中最长的。当女性成年人注射合成P1 x-PBAN，信息素的生产表现出最大的增加I小时注射后。RT-PCR筛选结果表明，P1 x-PBAN cDNA在所有检测的身体部位均有表达，其中在雌性成虫头部的表达量最高。RT-PCR产物分析表明，P1 x-PBAN序列是相同的，在所有检查的身体部位的性别。系统发育分析表明，P1 x-PBAN基因与其他PBAN基因的亲缘关系较远，表现为相对较低的相似性。(c)2005年爱思唯尔公司All rights reserved.
Pheromone biosynthesis activating neuropeptide (PBAN) produced in the subesophageal ganglion stimulates pheromone production in the pheromone gland. A cDNA isolated from female adult heads of the diamondback moth (Plutella xylostella (L.)) encodes 193 amino acids including PBAN, designated as P1x-PBAN, and four other neuropeptides (NPs): diapause hormone (DH) homologue, alpha-NP, beta-NP and gamma-NP. All of the peptides are amidated in their C-termini and shared a conserved motif, FXPR(or K)L structure, as reported from other PBAN cDNAs. P1x-PBAN consists of 30 amino acids, the shortest PBAN so far reported. P1x-PBAN exhibited below 50% homology, compared with other known PBANs. The P1x-DH homologue is structurally different from DH of Bombyx mori. The length of P1x-beta-NP (16 amino acids) was the shortest and showed relatively low similarity, whereas gamma-NP (10 amino acids in length) was the longest among examined gamma-NPs. When female adults were injected with synthetic P1x-PBAN, pheromone production showed a maximal increase I h post-injection. RT-PCR screening revealed that P1x-PBAN cDNA was expressed in all examined body parts, with the highest expression level in the head of female adults. Analysis of RT-PCR products indicated the P1x-PBAN sequence was identical in all examined body parts of both sexes. Phylogenetic analysis revealed that the P1x-PBAN gene is distantly related to other PBANs, demonstrated by the relatively low similarity. (c) 2005 Elsevier Inc. All rights reserved.