The organic matrix of the skeletal spicule of sea urchin embryos.
The organic matrix of the skeletal spicule of sea urchin embryos.
复制标题
海胆胚胎骨骼骨针的有机基质。
DOI：
10.1083/jcb.102.5.1878
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发表时间：
1986-05
期刊：
影响因子：
--
通讯作者：
Wilt F
中科院分区：
文献类型：
--
作者：
Benson SC;Benson NC;Wilt F
在海胆胚胎发育的第四次细胞分裂时出现的微粒体产生初级间充质，初级间充质继而分化并产生钙质内骨骼骨针。这些骨针已被分离和纯化，从pluteus幼虫洗涤离子和非离子洗涤剂的组合，然后短暂暴露于次氯酸钠。针状体可能脱矿，完整的基质溶解。基质由富含aspx、glux、gly、ser和ala的有限数量的糖蛋白组成，该组合物与在软体动物、海绵和节肢动物的生物矿化组织的基质蛋白中发现的组合物没有什么不同。没有证据表明胶原蛋白是基质的成分。基质含有复合物类型的N-连接糖蛋白。在骨针沉积发生期间，基质主要由原肠胚形成后期向前合成的蛋白质产生。蛋白质的混合物结合钙，是一种有效的免疫原。在含有SDS的丙烯酰胺凝胶上电泳糖蛋白，然后进行印迹和免疫细胞化学检测，揭示了约47，50，57和64 kD的主要成分，和几个次要成分。这些相同的组分可以用氨基酸标记蛋白质的银染色或荧光照相法检测。除了为研究微节谱系的发育提供方便的分子标记外，骨针基质糖蛋白还为研究生物矿化提供了一个有趣的系统。
The micromeres that arise at the fourth cell division in developing sea urchin embryos give rise to primary mesenchyme, which in turn differentiates and produces calcareous endoskeletal spicules. These spicules have been isolated and purified from pluteus larvae by washing in combinations of ionic and nonionic detergents followed by brief exposure to sodium hypochlorite. The spicules may be demineralized and the integral matrix dissolves. The matrix is composed of a limited number of glycoproteins rich in aspx, glux, gly, ser, and ala, a composition not unlike that found in matrix proteins of biomineralized tissues of molluscs, sponges, and arthropods. There is no evidence for collagen as a component of the matrix. The matrix contains N-linked glycoproteins of the complex type. The matrix arises primarily from proteins synthesized from late gastrulation onward, during the time that spicule deposition occurs. The mixture of proteins binds calcium and is an effective immunogen. Electrophoresis of the glycoproteins on SDS-containing acrylamide gels, followed by blotting and immunocytochemical detection, reveals major components of approximately 47, 50, 57, and 64 kD, and several minor components. These same components may be detected with silver staining or fluorography of amino acid-labeled proteins. In addition to providing convenient molecular marker for the study of the development of the micromere lineage, the spicule matrix glycoproteins provide an interesting system for investigations in biomineralization.