Advancement and applications of peptide phage display technology in biomedical science.
Advancement and applications of peptide phage display technology in biomedical science.
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DOI：
10.1186/s12929-016-0223-x
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发表时间：
2016-01-19
影响因子：
11
通讯作者：
Wu HC
中科院分区：
文献类型：
--
作者：
Wu CH;Liu IJ;Lu RM;Wu HC
组合噬菌体文库是高通量筛选蛋白质相互作用的有力工具。在所有可用的分子展示技术中，噬菌体展示已被证明是最流行的方法。筛选噬菌体随机肽库是筛选能与靶分子结合并调节其功能的肽的有效手段。噬菌体展示肽文库可用于（i）B细胞和T细胞表位作图，（ii）选择与受体或蛋白质结合的生物活性肽、疾病特异性抗原模拟物、与非蛋白质靶标结合的肽、细胞特异性肽或器官特异性肽，和（iii）开发肽介导的药物递送系统和其他应用。利用噬菌体展示技术鉴定的靶向肽可用于基础研究和转化医学。本文综述了噬菌体展示肽库在生物医学中的应用。
Combinatorial phage library is a powerful research tool for high-throughput screening of protein interactions. Of all available molecular display techniques, phage display has proven to be the most popular approach. Screening phage-displayed random peptide libraries is an effective means of identifying peptides that can bind target molecules and regulate their function. Phage-displayed peptide libraries can be used for (i) B-cell and T-cell epitope mapping, (ii) selection of bioactive peptides bound to receptors or proteins, disease-specific antigen mimics, peptides bound to non-protein targets, cell-specific peptides, or organ-specific peptides, and (iii) development of peptide-mediated drug delivery systems and other applications. Targeting peptides identified using phage display technology may be useful for basic research and translational medicine. In this review article, we summarize the latest technological advancements in the application of phage-displayed peptide libraries to applied biomedical sciences.