Amino-terminal extended peptide single-chain trimers are potent synthetic agonists for memory human CD8+ T cells.
Amino-terminal extended peptide single-chain trimers are potent synthetic agonists for memory human CD8+ T cells.
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DOI：
10.4049/jimmunol.1103647
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发表时间：
2012-06-15
期刊：
影响因子：
--
通讯作者：
Linette GP
中科院分区：
文献类型：
--
作者：
Carreno BM;Becker-Hapak M;Chan M;Lie WR;Wang X;Hansen TH;Linette GP
Upon antigen exposure, most memory T cells undergo re-stimulation induced cell death. Here we describe a novel synthetic agonist, an amino-terminal extended decamer peptide expressed as a single chain trimer, the AT-SCT, that preferentially promotes the growth of memory human CD8+ T cells with minimal re-stimulation-induced cell death. Using the CMV pp65 and melanoma gp100 antigens, we observe the in vitro numerical expansion of a clonally diverse poly-functional population of antigen-specific CD8+ T cells from normal individuals and vaccinated melanoma patients, respectively. Memory CD8+ T cells stimulated with AT-SCT presented on MHC class I/II null cells show reduced cytokine production, slower kinetics of TCR down-regulation and decreased cell death when compared to native nonamer SCT-activated T cells. However, both ERK phosphorylation and cell cycle kinetics are identical in AT-SCT- and SCT-activated T cells. Probing of SCT and AT-SCT peptide-MHC (p-MHC) complexes using fluorochrome-conjugated TCR multimers suggest that nonamer and decamer-linked peptides may be anchored differently to HLA-A2 peptide binding groove. Our findings demonstrate that modified p-MHC structures such as AT-SCT can be engineered as T cell agonists to promote the growth and expansion of memory human CD8+ T cells.
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影响因子：
15.3
作者：
Inaba, K;Steinman, R M
通讯作者：
Steinman, R M
影响因子：
5.6
作者：
Borbulevych, Oleg Y.;Insaidoo, Francis K.;Baker, Brian M.
通讯作者：
Baker, Brian M.
DOI：
10.1073/pnas.1110971108
发表时间：
2011-08-16
影响因子：
11.1
作者：
Samanta, Dibyendu;Mukherjee, Gayatri;Almo, Steven C.
通讯作者：
Almo, Steven C.
影响因子：
11.5
作者：
Linette, GP;Zhang, DS;Haluska, FG
通讯作者：
Haluska, FG
DOI：
10.1084/jem.20062363
发表时间：
2007-06-11
期刊：
The Journal of experimental medicine
影响因子：
--
作者：
Precopio ML;Betts MR;Parrino J;Price DA;Gostick E;Ambrozak DR;Asher TE;Douek DC;Harari A;Pantaleo G;Bailer R;Graham BS;Roederer M;Koup RA
通讯作者：
Koup RA