Development of an efficient plant dual cytosine and adenine editor
Development of an efficient plant dual cytosine and adenine editor
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DOI：
10.1111/jipb.13146
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发表时间：
2021-08-02
影响因子：
11.4
通讯作者：
Wei, Pengcheng
中科院分区：
文献类型：
--
作者：
Xu, Rongfang;Kong, Fanna;Wei, Pengcheng
从日本七鳃鳗CDA1-like 4中建立了一个增强型CDA-like (eCDAL)，作为c -末端胞嘧啶碱基编辑器（CT-CBE），在广泛的区域内实现了高编辑频率。然后，将TadA-8e集成到eCDAL中，开发了一种新的植物双碱基编辑器版本1（pDuBE1）。pDuBE1的编辑效率可达87.6%，在稳定转化的植物细胞中，A-to-G和C-to-T的同步转换频率高达49.7%。研究结果表明，pDuBE1可以介导植物基因组的双编辑，为作物的精确育种和植物直接进化的筛选提供了强大的操作工具。
An enhanced CDA-like (eCDAL) was established from Japanese lamprey CDA1-like 4 to achieve a high editing frequency in a broad region as a C-terminal cytosine base editors (CT-CBE). Then, a novel plant dual-base editor version 1(pDuBE1) was developed by integrating TadA-8e into eCDAL. The editing efficiency of pDuBE1 could reach to 87.6%, with frequencies of concurrent A-to-G and C-to-T conversions as high as 49.7% in stably transformed plant cells. Our results showed that pDuBE1 could mediate robust dual editing in plant genome, providing a powerful manipulation tool for precise crop breeding and screening platforms for in planta direct evolution.