Wide field-of-view microscope based on holographic focus grid illumination
Wide field-of-view microscope based on holographic focus grid illumination
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DOI：
10.1364/ol.35.002188
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发表时间：
2010-07-01
期刊：
影响因子：
3.6
通讯作者：
Yang, Changhuei
中科院分区：
文献类型：
--
作者：
Wu, Jigang;Cui, Xiquan;Yang, Changhuei
我们开发了一种新的显微镜设计，可以实现大视场(FOV)成像，同时具有与传统显微镜相当的分辨率。在我们的设计中，样品由全息投影的光斑网格照明。我们通过在网格中平移样本并检测传输来获取图像。我们搭建了一个视场为6 mm×5 mm的原型系统，采集时间为2.5 S。分辨率从根本上受到光斑大小的限制-我们展示的平均半高宽光斑直径为0.74微米。我们通过对一个美国空军目标和一个百合花药成像来演示这个原型。这项技术是可扩展的，是实现宽视野显微镜系统的一种经济高效的方式。(C)2010年美国光学学会
We have developed a new microscopy design that can achieve wide field-of-view (FOV) imaging and yet possesses resolution that is comparable to a conventional microscope. In our design, the sample is illuminated by a holographically projected light-spot grid. We acquire images by translating the sample across the grid and detecting the transmissions. We have built a prototype system with an FOV of 6 mm x 5 mm and acquisition time of 2.5 s. The resolution is fundamentally limited by the spot size-our demonstrated average FWHM spot diameter was 0.74 mu m. We demonstrate the prototype by imaging a U.S. Air Force target and a lily anther. This technology is scalable and represents a cost-effective way to implement wide FOV microscopy systems. (C) 2010 Optical Society of America